Toksisitas Short Chain Fatty Acid (SCFA), Produk Turunan Pati Resisten Tipe 3 Hasil Fermentasi Ubi Jalar (Ipomoea batatas) Oleh Bakteri Clostridium butyricum BCC B2571 Terhadap Sel HCT-116

Abstract

Sweet potato (Ipomoea batatas) is a food that contains high type 3 resistant starch. Fermentation of type 3 resistant starch by several bacteria such as Clostridium can produce Short Chain Fatty Acid (SCFA) containing acetate, propionate, and butyrate. SCFA butyrate is capable to inhibit the growth of colon carcinoma cells with an apoptosis pathway. The aim of this research was to analyze the toxicity of SCFA produce from fermentation of type-3 resistant starch from sweet potato with the bacterium Clostridium butyricum BCC B2571 against human colon carcinoma cell line using hemacytometer cell counting method and detection of apoptotic cells by Hoechst 33258 staining. The type 3 resistant starch fermented from sweet potato with a molar ratio of acetate, propionate, and butyrate at 52.95 mM : 66.93 mM : 92.41 mM were used as the primary working solution which was further diluted. VERO cell was used in the analysis as a normal cell with treatment given at concentration of 10 mM; 5 mM: 2.5 mM: 1.25 mM: 0.625 mM, while HCT 116 cells was used as a colon cancer cells by treatment with 1.25 mM; 0.625 mM: 0.313 mM: 0.156 mM; 0.078 mM. The results showed that the butyrate concentration of 1.25 mM showed the high toxicity of 85.42% to HCT 116 cells and in VERO cells showed low toxicity at 46.97%. Detection of apoptosis using Hoechst 33258 staining showed that apoptosis occurred in HCT-116 cells by a SCFA concentration of 1.25 mM and 0.625 mM.