Pengaruh Pemberian Bubuk Daun Cincau Hijau (Premna oblongifolia Merr) Terhadap Gambaran Histopatologis Jaringan Hati Mencit C3H yang Ditransplantasi Sel Tumor Kelenjar Susu

Abstract

Being the second of the biggest killer machine in the world, cancer still has a possibility to be prevented. Cancer curing usually involves radiotherapy and chemotherapy which have cytotoxic effects not only for the cancer cells but also for the normal cells. The best way to prevent cancer is by consuming natural food products. Natural foods such as fruits and vegetables have been proved to have many benefit effects to body health. Cincau hijau extracts had been proved to have anticancer activity; to be toxic to cancer cells but not toxic to normal cells according to in vitro researchs. The in vivo anticancer activities of cincau hijau Premna oblongifolia Merr was examined in this study by using C3H mice. The dosages of cincau hijau leaf powder given to the mice were 0.88%, 1.76%, and 2.64% in powder form. The 2.64% dosage had a decreasing effect in mice body weight. Tumor volume increased significantly in the negative control group, especially eleven days after the tumor cell transplantion. Mice tumor latency period and liver weight were not significantly different (p>0.05). Tumor tissue weights of negative control and 0.88% group were significantly larger than the others (p<0.05). Hepatocytes lesions were observed histologically using hematoxylin-eosin staining process and counted in percentage and statistical score. The percentage of normal hepatocytes of the negative control was the highest (3.18%). The percentage of hydropic degeneration of 0.88% group was the highest (30.66%). Hepatocytes lesions by hydropic degeneration between negative control and the 0.88%, 1.76%, and 2.64% groups were not significantly different (p>0.05). The percentage of fatty degeneration of the 2.64% group was the highest (77.76%). Hepatocytes lesions by fatty degeneration between positive, negative control, and the 0.88% were not significantly different (p>0.05). The percentage of necrosis of the 0.88% group was the highest (11.05%). Hepatocytes lesions by necrosis between negative control and the 1.76% and 2.64% groups were not significantly different (p>0.05), and the hepatocytes lesions by necrosis of 0.88% group was significantly larger than the others (p<0.05). Liver degeneration might be caused by the tumor existence and growth which continue to affect liver metabolism. The 0.88% dosage had not shown effective protection to liver injury. It might be caused by the amount of feed consumption which was not significantly different (p>0.05) and by the tumor.