Hemolisis Eritrosit In Vitro Serta Kapasitas Antioksidan Pepes Ikan Mas Iradiasi Dan Non Iradiasi
Abstract
A variety methods of food preservation have been developed to produce products with maximum storage period, such as drying, pasteurisation, sterilisation, and canning. Those methods need high temperature processing, that can cause degradation of food nutritions, for example protein and vitamine. Those methods also can cause organopleptic quality alteration in foods, like colour, taste, and aroma. Irradiation method is alternative of food preservation method that is done by exposing food with controling ionisation radiation certain interval time to attain the definite purpose. This method does not apply high temperature, but, in fact, many people still doubt about the safety of irradiation products may cause negative effect for human health. The aim of this research is definite the safety of irradiation fish pepes by observing free radical activity in irradiated pepes product in vitro by erythrocyte hemolysis and antioxidant capacity. This research can be devided into four steps; samples preparation consisting ofthe making of irradiation gold fish pepes extract, chemical analysis (DPPH assay), the hemolysis rate of erythrocytes measurement, and statistic testing with ANOVA methode. The hemolysis rate of erythrocytes in vitro and antioxidant capacity measurments of irradiated gold fish pepes indicated that samples with period of storage more than 12 month (November 11th 2006 pepes and June 14th 2007 pepes) have higher hemolysis rate of erythrocytes and lower antioxidant capacity than samples with storage period less than 12 month (April 5th 2008 pepes and No Label pepes). The antioxidant capacity of November 11th 2006 Rell Ion pepes is 21.06%, and the hemolysis rate of erythrocytes is 129.14%. The antioxidant capacity of November 14th 2007 Dipa pepes is 20.42%, and the hemolysis rate of erythrocytes is 125.11%. The antioxidant capacity of April 5th 2008 pepes is 31.91%, and the hemolysis rate of erythrocytes is 114.20%. The antioxidant capacity of No Label pepes is 33.83%, and the hemolysis rate of erythrocytes is 114.20%. The antioxidant capacity of control pepes is 15.53%, and the hemolysis rate of erythrocytes is 95.96%. The influence of sample dilution in this research is tended to increase the rate hemolysis of erythroctes. Based on statistic test ANOVA method, control pepes is not real different with irradiated pepes. In addition, there were no difference at 0.05 invariance of interval..