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      • UT - Aquatic Product Technology
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      Identifikasi dan karakterisasi bakteri vibrio parahaemolyticus pada produk perikanan

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      Date
      2021-08
      Author
      Ismail
      Sumaryanto, Heru
      Abdullah, Asadatun
      Kusmarwati, Arifah
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      Abstract
      Vibrio merupakan genus bakteri gram negatif, sel tunggal berbentuk batang pendek yang bengkok, berukuran panjang 1,4-5,0 µm dan lebar 0,3-1,3 µm, bersifat motil dan mempunyai flagel polar dan secara khas ditemukan pada air laut. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi kandungan bakteri V. parahaemolyticus pada produk perikanan. Metode uji yang digunakan dalam penelitian ini menggunakan beberapa metode yaitu, uji agar selektif yang mendapatkan hasil empat sampel negatif dan 16 sampel positif terdeteksi Vibrio. Hasil uji ketahanan suhu dan ketahanan garam mendapatkan hasil dua negatif dan 14 hasil positif. Hasil uji oksidasi mendapatkan hasil empat hasil negtif dan 10 hasil positif. Hasil uji API 20E terdeteksi 6 isolat yang mengandung V. parahaemolyticus dan delapan isolat tidak terdeteksi mengandung V. parahaemolyticus. Hasil uji cepat menunjukkan isolat yang teridentifikasi bakteri V. parahaeolyticus adalah SCU, SCC, SLKD, SLB, SRBL dan SRU isolat yang tidak teridentifikasi bakteri yaitu SCKB, SCB, SLU, SBB, SBKTG, SBU, SRBLA dan SRKD.
       
      Vibrio is a genus of gram-negative bacteria, single-cell bent short rods, measuring 1.4 - 5.0 µm in length and 0.3 - 1.3 µm in width, are motile and have polar flagella and are typically found in seawater. This study aims to isolate and identify the content of V. parahaemolyticus bacteria in fishery products. The test method used in this study uses several rapid test methods, including the test to be selective to get four negative samples and 16 positive samples detected by vibrio. The test results of temperature resistance and salt resistance get two negative results and 14 positive results. The results of the oxidation test got four negative results and 10 positive results. API 20E test results detected 6 isolates containing V. parahaemolyticus and eight isolates were not detected containing V. parahaemolyticus. Rapid test results showed the isolates identified by the Vibrio parahaeolyticus bacteria were SCU, SCC, SLKD, SLB, SRBL and SRU isolates that were not identified by bacteria, namely SCKB, SCB, SLU, SBB, SBKTG, SBU, SRBLA and SRKD
       
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      http://repository.ipb.ac.id/handle/123456789/108462
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      • UT - Aquatic Product Technology [2462]

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      Copyright © 2020 Library of IPB University
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      Indonesia DSpace Group 
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      Universitas Jember Digital Repository