Formula Optimization of Meat Analog Derived from Pleurotus Flabellatus Mycelium by Response Surface Methodology

Abstract

Mushroom was known as food with high protein content. It has been widely consumed in Indonesia for example is oyster mushroom or Pleurotus spp. Pleurotus flabellatus (pink oyster mushroom) mycelium was potential ingredient of meat substitute for people who avoid meat consumption. The objective of this research was to determine the optimum formula of meat analog derived from P. flabellatus mycelium by response surface methodology (RSM). Meat analog was produced by steaming method. Other ingredient such as soybean flour, tapioca starch, and sweet potato flour was added in meat analog formulation. The product of meat analog was selected based on similar textural properties to those found in meat. This research resulted that the product consist of 40% mycelium, 30% soybean flour, 20% tapioca starch, and 10% sweet potato flour. It contains 50.14% carbohydrate, 19.30% protein, 27.50% fat, 3.06% ash, 10.50% dietary fiber, 14.67 mgGAE/ g total phenolic capacity, DPPH (6.27 mg AEAC/g product) and FRAP (13.47 mg FeSO4/ g product), glycemic index (20.06) and glycemic load (10.06). It also contains nine essential amino acid and high of unsaturated fatty acid.