Sakarifikasi Empulur Sagu (Metroxylon sagu) dengan Konsorsium Enzim Amilolitik dan Holoselulolitik untuk Produksi Bioetanol

Abstract

Starchy materials such as sago in bioethanol production is one effort in finding solutions to the depletion of fossil fuel reserves but leaving a large amount of wastes. In this research, native sago pith was used directly for bioethanol production. Sago pith is heterogeneous substrates that need pretreatment to reduce the level of crystallinity of fibers and dissolve the starch; also required a number of enzymes to hydrolyze. This research aim is to determine the effects of microwave treatment and consortia of enzymes (α-amylase, amyloglucosidase and pullulanase, cellulase and xylanase) in fermentable sugar production process from the pith of sago palm and its utilization for bioethanol production. This study included the preparation of raw materials sago pith, pith composition analysis including proximate components, starch, amylose and the composition of fiber (cellulose, hemicellulose, and lignin), characterization and preparation of enzymes for determining the optimum temperature, the process of hydrolysis and characterization of syrup. Furthermore, the results of hydrolysis are used in the production of bioethanol in batch and closed systems. The results showed that, microwave treatment made starch easy to swell and break the crystalline structure of fiber. Enzymes consortia in saccharification increased the fermentable sugar production but S. elippsoides used tend to convert glucose into organic acids in spite ethanol.