Identifikasi Single Nucleotide Polymorphism (SNP) Daerah 3’UTR Gen Tyrosinase (TYR) pada Sapi Bali

Abstract

Daerah 3’UTR dikenal sebagai pengatur penting terhadap ketepatan ekspresi pada banyak gen. Salah satu gen yang dapat menentukan warna bulu pada sapi bali adalah gen tyrosinase (TYR). SNP atau single nucleotide polymorphism merupakan variasi basa tunggal yang dapat menyebabkan keragaman pada sebuah populasi individu dari spesies yang sama. Tujuan penelitian adalah untuk mengidentifikasi dan menentukan SNP di daerah 3

The 3'UTR region is an important regulator of expression of many genes. One of the genes that can determine coat color in bali cattle is the TYR gene. SNP is a single base variation that can cause diversity in a population of individuals of the same species. This study aimed to to identify and determine SNPs in the 3'UTR region of the TYR gene in normal hair-colored bali cattle and albino bali cattle. A total of 6 albino cattle and 7 normal bali cattle were analyzed using the PCR method, electrophoresis analysis and then direct sequencing. Sequence data were analyzed using the MEGA10 program. The results of the analysis of the TYR gene in the 3'UTR region found 6 SNPs, namely SNP c.1684G>A, c.1687T>G, c.1702G/-, c.1737T>C, c. 1785A>G, and c.1809C>T. Based on the results obtained, the SNP found in the 3'UTR area can differentiate between normal and albino hair color bali cattle. SNPs c.1684G>A, c.1687T>G, c.1702G/-, c.1737T>C, c. 1785A>G, and c.1809C>T can be used as candidates for characterizing albino and normal hair color in bali cattle