Isolasi dan Identifikasi Bakteri Patogen pada Glass Eel Anguilla bicolor yang Dipelihara di Departemen Budidaya Perairan, IPB
Abstract
Eel (Anguilla bicolor) is a catadromous fish with high business prospects in
the field of cultivation in both domestic and international markets. National eel
production experienced a decline in 2020 by an average of 37.64%. A decrease in
eel production can occur due to production failure due to disease. This research
aims to isolate and identify pathogenic bacteria that cause disease and death in glass
eel Anguilla bicolor kept at the Department of Aquaculture, IPB. This research
method includes sampling, sample collection, observing clinical symptoms,
isolating bacteria, identifying bacteria, examining bacteria through Polymerase
Chain Reaction (PCR) analysis, and histopathology. The results of the research
showed that there were 10 bacterial isolates that dominantly grew, including
Aeromonas sp, Clostridium sp, Listeria sp, Bacillus sp, Staphylococcus sp,
Eubacterium sp, Micrococcussp. Chromobacterium sp, Vibrio sp, and Plesiomonas
sp. The five bacterial isolates were further tested using PCR and Aeromonas
hydrophila bacteria were identified. Histopathology results showed pathological
microanatomical changes in the gill, kidney, liver and intestine tissue showing
necrosis, edema, congestion, telangiectasis and villous abnormalities. The dominant
pathogenic bacteria found and potentially causing disease in glass eel rearing in the
Aquaculture Department is Aeromonas hydrophila, characterized by clinical
symptoms of redness of the gills and damage to several tissues such as the gills,
liver, kidneys and intestines.
Collections
- UT - Aquaculture [2014]