Isolasi dan Identifikasi Bakteri Patogen pada Glass Eel Anguilla bicolor yang Dipelihara di Departemen Budidaya Perairan, IPB

Abstract

Eel (Anguilla bicolor) is a catadromous fish with high business prospects in the field of cultivation in both domestic and international markets. National eel production experienced a decline in 2020 by an average of 37.64%. A decrease in eel production can occur due to production failure due to disease. This research aims to isolate and identify pathogenic bacteria that cause disease and death in glass eel Anguilla bicolor kept at the Department of Aquaculture, IPB. This research method includes sampling, sample collection, observing clinical symptoms, isolating bacteria, identifying bacteria, examining bacteria through Polymerase Chain Reaction (PCR) analysis, and histopathology. The results of the research showed that there were 10 bacterial isolates that dominantly grew, including Aeromonas sp, Clostridium sp, Listeria sp, Bacillus sp, Staphylococcus sp, Eubacterium sp, Micrococcussp. Chromobacterium sp, Vibrio sp, and Plesiomonas sp. The five bacterial isolates were further tested using PCR and Aeromonas hydrophila bacteria were identified. Histopathology results showed pathological microanatomical changes in the gill, kidney, liver and intestine tissue showing necrosis, edema, congestion, telangiectasis and villous abnormalities. The dominant pathogenic bacteria found and potentially causing disease in glass eel rearing in the Aquaculture Department is Aeromonas hydrophila, characterized by clinical symptoms of redness of the gills and damage to several tissues such as the gills, liver, kidneys and intestines.