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      Minyak Hati Ikan Cucut (Centrophorus sp.) Diperkaya dengan Konsentrat Omega-3 sebagai Imunostimulan

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      Date
      2023
      Author
      Siregar, Mariana Kristin
      Suseno, Sugeng Heri
      Ramadhan, Wahyu
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      Abstract
      Minyak hati ikan cucut kaya akan squalene namun rendah omega-3. Squalene dan omega-3 diketahui masing-masing memiliki efek imunostimulan. Penelitian terkait pengaruh pemberian minyak ikan mengandung komponen squalene dan omega yang tinggi terhadap imunitas belum pernah dilakukan. Penelitian ini menggunakan minyak hati ikan cucut yang ditambahkan dengan konsentrat omega- 3 pada konsentrasi tertentu. Penambahan omega-3 pada minyak hati cucut akan meningkatkan kandungan asam lemak tak jenuh pada minyak ikan yang dapat mengakibatkan minyak ikan lebih mudah mengalami oksidasi. Penambahan antioksidan dapat memperpanjang masa simpan produk. Penelitian ini bertujuan menganalisis pengaruh penambahan konsentrat omega-3 pada minyak hati cucut sebagai imunostimulan melalui uji in vitro dan mengetahui pengaruh konsentrasi antioksidan terhadap oksidasi minyak ikan selama penyimpanan. Penambahan konsentrat omega-3 pada minyak hati cucut menunjukkan efek imunostimulan yang lebih tinggi dibandingkan minyak kontrol berdasarkan nilai viabilitas splenosit dan fagositosis makrofag. Penambahan antioksidan pada minyak dapat menghambat oksidasi minyak hati cucut selama penyimpanan hingga 30 hari pada suhu ruang untuk semua sampel, namun pada konsentrasi 6000 ppm terjadi prooksidan yang diakibatkan konsentrasi antioksidan yang ditambahkan terlalu tinggi.
       
      Shark liver oil is rich in squalene but low in omega-3. Squalene and omega- 3 are known to each have an immunostimulating effect. Research related to the effect of fish oil containing high squalene and omega components on immunity has never been carried out. This study used shark liver oil added with omega-3 concentrate at a certain concentration. The addition of omega-3 to shark liver oil will increase the content of unsaturated fatty acids in fish oil which can make fish oil more susceptible to oxidation. The addition of antioxidants can extend the shelf life of the product. This study aims to analyze the effect of adding omega-3 concentrate to shark liver oil as an immunostimulant through in vitro tests and to determine the effect of antioxidant concentrations on oxidation of fish oil during storage. The addition of omega-3 concentrate to the shark liver oil showed a higher immunostimulating effect than the control oil based on the values of splenocyte viability and phagocytosis of macrophages. The addition of antioxidants to oil can inhibit oxidation of shark liver oil during storage up to 30 days at room temperature for all samples, but at a concentration of 6000 ppm pro-oxidants occur due to the concentration of antioxidants added being too high.
       
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      http://repository.ipb.ac.id/handle/123456789/126707
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