dc.contributor.advisor | Abdullah, Asadatun | |
dc.contributor.advisor | Nurilmala, Mala | |
dc.contributor.author | Putri, Annisaa | |
dc.date.accessioned | 2023-08-10T06:30:34Z | |
dc.date.available | 2023-08-10T06:30:34Z | |
dc.date.issued | 2023 | |
dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/123512 | |
dc.description.abstract | Permintaan pasar internasional terhadap hiu yang semakin meningkat,
mengakibatkan angka penangkapan beberapa spesies hiu semakin meningkat pula.
Spesies Prionace glauca dan Carcharhinus spp. termasuk spesies hiu dilindungi
yang masuk dalam daftar IUCN redlist dan Apendix II CITES. Autentikasi
menggunakan biomolekuler dengan memanfaatkan DNA perlu dilakukan.
Penelitian ini bertujuan membuat primer spesifik secara in silico berbasis gen
marka COI dan cyt b pada kelompok hiu yang terancam punah (Prionace glauca
dan Carcharhinus spp.) serta aplikasinya secara in vitro untuk autentikasi produk
hasil perikanan dengan teknik real-time PCR. Penelitian dilakukan dengan
merancang desain primer spesifik spesies target, preparasi sampel, isolasi DNA,
amplifikasi DNA, pengujian kualitas dan kuantitas DNA, dan analisis
menggunakan real-time PCR. Perancangan primer spesies target Prionace glauca
marka gen cyt b dan primer degenerate Carcharhinus spp. marka gen COI
berhasil dilakukan secara in silico. Efisien kondisi real-time PCR sampel
Prionace glauca dan Carcharhinus spp. telah sesuai dengan standar pengujian
menggunakan metode qPCR. | id |
dc.description.abstract | International market demand for sharks is increasing, resulting in the
number of catches of several shark species also increasing. Prionace glauca and
Carcharhinus spp. including protected shark species that are included in the
IUCN redlist and CITES Appendix II. Authentication using biomolecular by
utilizing DNA is necessary. This study aims to produce in silico specific primers
based on COI and cyt b marker genes for endangered sharks (Prionace glauca and
Carcharhinus spp.) and to apply them in vitro to authenticate fishery products
using real-time PCR techniques. The research was carried out by designing target
species-specific primers, sample preparation, DNA isolation, DNA amplification,
DNA quality and quantity testing, and analysis using real-time PCR. Primary
design of target species Prionace glauca marker cyt b gene and degenerate primer
Carcharhinus spp. COI gene markers were successfully carried out in silico.
Efficient real-time PCR conditions of Prionace glauca and Carcharhinus spp.
complies with testing standards using the qPCR method. | id |
dc.description.sponsorship | Dana penelitian dari PTKN Kemendikbudristek tahun anggaran 2022 atas nama Dr. Asadatun Abdullah nomor kontrak 3876/IT3.L1/PT.01.03/P/B/2022. | id |
dc.language.iso | id | id |
dc.publisher | IPB University | id |
dc.title | Desain Primer Spesifik untuk Deteksi Pemanfaatan Spesies Hiu Prionace glauca dan Carcharhinus spp. dengan Metode RealTime PCR | id |
dc.title.alternative | Specific Primary Design for Detection of Shark Species Utilization Prionace glauca and Carcharhinus spp. Using Real-Time PCR Method | id |
dc.type | Undergraduate Thesis | id |
dc.subject.keyword | CITES | id |
dc.subject.keyword | hiu | id |
dc.subject.keyword | in silico | id |
dc.subject.keyword | IUCN redlist | id |
dc.subject.keyword | primer spesifik | id |
dc.subject.keyword | shark | id |
dc.subject.keyword | specific primer | id |