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      Kelangsungan Hidup Udang Vaname Yang Diberikan Rekombinan Viral Inhibitor Protein Melalui Pakan Dengan Frekuensi Berbeda Terhadap Infeksi WSSV

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      Date
      2023-02-02
      Author
      Qoyyim, Teuku Ibnul
      Alimuddin
      Arfah, Harton
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      Abstract
      White spot syndrome virus (WSSV) merupakan patogen yang sangat sering menginfeksi udang. Penelitian ini bertujuan menguji efektivitas rekombinan viral inhibitor protein (VIP) melalui pakan dengan frekuensi berbeda terhadap tingkat kelangsungan hidup udang vaname yang diuji tantang dengan WSSV. Penelitian ini dilakukan pada bulan Agustus-Oktober 2022 di Labroratorium Marikultur, analisis respons imun di Laboratorium Reproduksi dan Genetika Ikan, Departemen Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor. Penelitian ini dilaksanakan secara eksperimental menggunakan rancangan acak lengkap. Penelitian dilakukan menggunakan empat perlakuan dengan lima ulangan untuk uji tantang. Parameter uji yang diukur yakni tingkat kelangsungan hidup (TKH), total haemocyte count (THC), dan aktivitas phenoloxidase (PO). Data diolah menggunakan Microsoft Excel 2010 dan dianalisis dengan analisis ragam (ANOVA). Hasil penelitian ini menunujukkan bahwa pemberian VIP dengan frekuensi setiap hari memberikan hasil terbaik setelah diinfeksikan dengan WSSV yang menghasilkan tingkat kelangsungan hidup mencapai 45,33% dibandingkan (P<0,05) kontrol positif yang memiliki tingkat kelangsungan hidup terendah yakni 9,33%.
       
      White spot syndrome virus (WSSV) is a pathogen that often infects shrimp. This study aims to test the effectiveness of recombinant viral inhibitor protein (VIP) by feeding with different frequencies on the survival rate of vannamei shrimp challenged with WSSV. This research was conducted in August-October 2022 at Mariculture Laboratory, immune response analysis at Fish Reproduction and Genetics Laboratory, Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University. This research was carried out experimentally using a completely randomized design. The study was conducted using four treatments with five replications for the challenge test. The test parameters measured were survival rate (TKH), total haemocyte count (THC), and phenoloxidase (PO) activity. Data were processed using Microsoft Excel 2010 and analyzed by analysis of variance (ANOVA). The results of this study showed that giving VIP with a daily frequency gave the best results after being infected with WSSV which resulted in a survival rate of 45.33% compared to (P<0.05) the positive control which had the lowest survival rate of 9.33%.
       
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      http://repository.ipb.ac.id/handle/123456789/116668
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      • UT - Aquaculture [2189]

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      Indonesia DSpace Group 
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