dc.contributor.advisor | Gunawan, Asep | |
dc.contributor.advisor | Sumantri, Cece | |
dc.contributor.author | Astira, Adinda Wanda | |
dc.date.accessioned | 2022-07-19T06:36:50Z | |
dc.date.available | 2022-07-19T06:36:50Z | |
dc.date.issued | 2022 | |
dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/112614 | |
dc.description.abstract | Selenoprotein W1 (SEPW1) adalah protein yang mengandung selenium yang
memiliki peran penting dalam pertumbuhan dan perkembangan otot melalui
perlindungan myoblast dari stres oksidatif. Penelitian ini bertujuan untuk
mengidentifikasi keragaman gen SEPW1 dan hubungannya dengan kandungan mineral
pada domba. Sampel yang digunakan sebanyak 40 ekor domba yang terdiri dari 10
domba ekor tipis (DET), 10 domba compass agrinak (DCA), 10 domba garut (DG),
dan 10 domba jonggol (DJ). Kandungan mineral yang terpilih ditentukan dengan
menggunakan metode IK LP-04.10-LT-1.0. Identifikasi polimorfisme gen SEPW1
dilakukan menggunakan Polymerase Chain Reaction-Restriction Fragment Length
Polymorphism (PCR-RFLP). Ukuran produk amplifikasi adalah 455 bp. Uji chi
kuadrat menunjukkan populasi yang diamati berada dalam keseimbangan Hardy
Weinberg. Keragaman gen SEPW1 ditemukan pada setiap rumpun domba. Hasil
analisis menunjukkan SNP g.53617063 A>G gen SEPW1 berasosiasi secara
signifikan (P<0.05) dengan kandungan mineral selenium. Gen SEPW1 berpotensi
digunakan sebagai penanda seleksi terhadap kandungan mineral pada domba | id |
dc.description.abstract | Selenoprotein W1 (SEPW1) is a protein containing selenium that has an
important role in muscle growth and development through protecting myoblasts
from oxidative stress. This study was aim to identify polymorphism of the SEPW1
gene and its association with mineral content in sheep. The samples used were 40
sheep consisting 10 samples of javanese thin tailed sheep (JTT), 10 samples of
compass agrinak sheep (CAS), 10 samples garut sheep (GS), and 10 samples of
jonggol sheep (JS). Selected mineral contents were determined using the IK LP 04.10-LT-1.0 method. Identification of SEPW1 gene polymorphism were
conducted using Polymerase Chain Reaction-Restriction Fragment Length
Polymorphism (PCR-RFLP). The size of the amplification product were 455 bp.
Chi-square analys showed that the population was in Hardy-Weinberg equilibrium.
Polymorphism of SEPW1 gene were found in each breed of sheep. A SNP
g.53617063 A>G of the SEPW1 gene were significantly associated (P<0.005) with
selenium mineral content. The SEPW1 gene was potential to be used as a selection
marker for mineral content in lamb. | id |
dc.language.iso | id | id |
dc.publisher | IPB University | id |
dc.title | Keragaman Gen Selenoprotein W1 (SEPW1) dan Asosiasinya terhadap Kandungan Mineral pada Daging Domba | id |
dc.title.alternative | Selenoprotein W1 (SEPW1) Gene Polymorphism and Association with Mineral Content in Lamb | id |
dc.type | Undergraduate Thesis | id |
dc.subject.keyword | Mineral Content | id |
dc.subject.keyword | PCR-RFLP | id |
dc.subject.keyword | SEPW1 Gene | id |
dc.subject.keyword | Sheep | id |