| dc.contributor.advisor | Gunawan, Asep | |
| dc.contributor.advisor | Sumantri, Cece | |
| dc.contributor.author | Azizah, Aisyah Nur | |
| dc.date.accessioned | 2021-07-30T12:56:35Z | |
| dc.date.available | 2021-07-30T12:56:35Z | |
| dc.date.issued | 2021 | |
| dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/107982 | |
| dc.description.abstract | Gen Nudix Hydrolase 8 (NUDT8) memiliki peran penting dalam
pertumbuhan kanker, khususnya CoA terhidrolisis, enzim mitokondria dan banyak
terekspresi dalam berbagai organ mitokondria seperti jantung, ginjal, hati, dan juga
jaringan adiposa coklat (BTA). Penelitian ini bertujuan untuk mengidentifikasi gen
NUDT8 dan hubungannya dengan sifat asam lemak. Jumlah sampel yang digunakan
adalah 63 ekor ayam ras pedaging (KBKB). Identifikasi gen NUDT8 dianalisis
menggunakan Polymerase Chain Reaction - Restriction Fragment Length
Polymorphism (PCR-RFLP). Hasil penelitian menunjukkan bahwa gen NUDT8
bersifat polimorfik yang ditunjukkan dengan adanya tiga genotipe pada gen NUDT8
(CC, CT, dan TT). Keragaman genotipik dari alel NUDT8 berada di Hardy
Weinberg Equilibrium. Analisis SNP g.372602 C> T, gen NUDT8 diasosiasikan
secara signifikan (P> 0,05) dengan Asam Lemak Jenuh (SFA), khususnya Asam
Palmitat (C16: 0). Gen NUDT8 bersifat polimorfik dan dapat digunakan sebagai
kandidat penanda komposisi asam lemak pada ayam ras broiler (KBKB). | id |
| dc.description.abstract | Nudix Hydrolase 8 (NUDT8) gene had an important role in cancer growth,
specifically hydrolyzed CoA, a mitochondrial enzyme and widely expressed in
mitochondrial diverse organs as a heart, kidney, liver, and also brown adipose tissue
(BTA). This research aimed to identify the NUDT8 gene and its association with
fatty acid traits. The total sample used was 63 broiler cross-breed chickens (KBKB).
Identification of NUDT8 gene were analyzed by using Polymerase Chain Reaction
- Restriction Fragment Length Polymorphism (PCR-RFLP). The results showed
that NUDT8 gene were polymorphic, which was indicated by presence of three
genotypes in the NUDT8 (CC, CT, and TT) gene. The genotyipic diversity of the
NUDT8 allels was in Hardy Weinberg Equilibrium. The analyses on SNP g.372602
C>T, NUDT8 gene was assosiated significantly (P>0.05) with Saturatted Fatty
Acid (SFA), specially of Palmitat Acid (C16:0). NUDT8 gene is polymorphic and
can be utilized as a candidate marker for fatty acids composition in broiler crossbreed chickens (KBKB). | id |
| dc.language.iso | id | id |
| dc.publisher | IPB University | id |
| dc.title | Identifikasi Keragaman dan Asosiasi Gen NUDT8 terhadap Asam Lemak pada Persilangan Ayam Kampung dan Ayam Tipe Pedaging | id |
| dc.title.alternative | Identification of Variety and Association of Fatty Acids in NUDT8 Gene with Native Chicken and Broiler Crossbreed | id |
| dc.type | Article | id |
| dc.type | Undergraduate Thesis | id |
| dc.type | Thesis | id |
| dc.type | Dissertation | id |
| dc.type | Student Paper | id |
| dc.subject.keyword | fatty acids | id |
| dc.subject.keyword | NUDT8 gene | id |
| dc.subject.keyword | PCR-RFLP | id |
