Induksi Maturasi Ikan Sidat (Anguilla bicolor) dengan Menggunakan Kombinasi Hormon Berbeda

Abstract

Production of Indonesian eel (Anguilla bicolor) juvenile through the hatchery can not be done yet. In this research, induction of Indonesian eel gonadm aturation was performed by hormonal with a combination of pregnant mare serum gonadotropin (PMSG), human chorionic gonadotropin (HCG) antidopamin and recombinant growth hormone (rGH). This research consisted of five treatments namely: control(NaCl 0,9%), PMSG 20 IU/kg, PMSG 20 IU/kg + antidopamin 10 ppm/kg, PMSG 20 IU/kg + antidopamin 10 ppm/kg + rGH 10 μg/kg dan PMSG 20 IU/kg + HCG 10 IU/kg. Each treatment contained 10 fishes. Hormonal induction was conducted by intramuscular injections, as much as five times at intervals of seven days. Furthermore observations on gonadal development were performed after injection for 21 days.The results showed that the treatment generated pregnancy level of 100%, while control was 0%. The best treatment is PMSG 20 IU/kg + antidopamin 10 ppm/kg+ rGH 10 μg/kg, seen from a more mature phase of the gametes, spermatocytes in male and oocytes with perinukleolar phase in female fish. Eel fish at body weight 120.4 to 207.8 g and 40.9 to 43.1 cm of body lenght was male, body weight of 274.8 g and length 47 cm was the intersexual phase eels, while at body weight 323.4 g and length 53 cm was female.