Sorgum (Sorghum bicolor L. Moench) Administra-tion Inhibit Colon Cancer Development in Balb/c Mice through Improvement of Colon Microenvironment.

Abstract

Sorgum (Sorghum bicolor L. Moench) contains dietary fibers and phenolic compounds that have been shown to have inhibitory effect on colon cancer development. The objectives of this research were to evaluate the effects of 50% polished sorgum administration in mouse diet to observe colon cancer development of AOM-DSS induced Balb/c mice. Male BALB/c mice (n = 32) were divided into 4 groups of 8. The carbohydrate source of group K- and K+ was cornstarch, for S50 group was 50% sorghum and 50% cornstarch, and for S100 group was 100% sorghum. Single intraperitoneal injection of AOM (10 mg/kg body weight) and administration of 1% DSS in drinking water for 7 days was conducted for colon carcinogenesis of group K+, S50, and S100. The results showed that administration of sorgum (S50 and S100) could inhibit colon carcinogenesis. Group S50 and S100 had lower β-glucoronidase activity and higher expression of caspase-3. These results were supported by histopathological profile improvement of colon, liver, and kidney, and also the diet consumption of S50 and S100 that was higher than those of K+. In addition, S100 group had a higher amount of fecal pH reduction, total short chain fatty acid, and total butyrate than S50 and K+. However, sorgum administration in S50 and S100 groups did not statistically affect CD4 expression. These indicate that administration of 100% sorgum in group S100 is better to protect against colon cancer development than group S50.