Efektivitas Isolat Bakteri Rumen Kerbau Pencerna Serat dalam Fermentasi Hijauan Leguminosa dan Pemanfaatan Urea in vitro

Abstract

Ruminants have ability to digest fiber component of feed due to the presence of cellulolitic bacteria in their rumen that secrete cellulase. Other bacteria have capability to digest protein by secreting protease and utilize non protein nitrogen. Fiber-digesting bacteria isolated from rumen of buffalo have not been confirmed in their ability to digest protein rich feed and utilize protein. This study aimed to evaluate the ability of fiber-digesting bacteria isolates in digesting leguminous herbage, degrading protein and utilizing non protein nitrogen. The first study was to compare the fermentability and coeficient digestibility of Calliandra callotyrsus, Leucaena leucocephala, Indigofera sp. and Gliricidia sepium by using bacteria in fresh rumen fluid and fiber-digesting bacteria isolates. The second study aimed to evaluate the ability of buffalo rumen bacterial isolates in the use of urea. Corn and cassava waste were used as carbohydrate sources with addition of urea as non protein nitrogen at level of 3%, 4,5%, and 6%. The first study showed that source of bacteria had no different effect on VFA concentration, and the coeficient digestibility of dry and organic matter of the herbage. The NH3 levels in the treatment of bacterial isolates was higher than that of fresh rumen fluid (P <0.05). The buffalo rumen bacterial isolates have the ability to degrade proteins and also showed that Indigofera sp., and Gliricidia sepium protein more susceptible to degradation than other legumes. The levels of NH3 in fermentaion media of feed added urea to 6% was in normal level. It was concluded that fiber-digesting rumen bacteria isolates were able to digest leguminous herbage and utilize the protein component of legume and capable of utilizing urea up to 6% in starchy feed.