Mekanisme aktivitas antitumor bubuk daun cincau hijau (Premna blongifolia Merr.) pada mencit c3h yang ditransplantasi sel tumor payudara

Abstract

This research aims to study the effects of antitumor activity of green gel leaf (Premna oblongifolia Merr.) powder on breast tumor cells transplanted C3H mice. Chemical analysis of the powder and histopathological analysis of the breast tumor tissue of C3H mice were performed. The chemical analysis consist of phytochemical analysis and antioxidant activity by free radical scavenger DPPH method. The positive phytochemical compound detection on the powder consists of alkaloids, saponins, phenol hydroquinones, molisch, benedict and tanins. The antioxidant activity by free radical scavenger DPPH method showed that the IC50 Keywords:C3H mice, green gelatin leaves powder dose, HE, CD31, caspase-3 of the powder methanolic extract was 16,90 ppm on the highest inhibition percentage (90,52%) on the 200 ppm concentration of the powder methanolic extract. The histopathological methods consist of haematoxylin-eosin (HE) and immunohistochemical (IHC) staining. The result showed that the best dose of the powder on the feed was the 2,64%. It was supported by the HE and IHC scores. The HE staining showed that the tumor tissues of the mice groups with 2,64% powder on the feed has the least average differentiation scores. It means that the tumor tissues has least change morphologically by the cell mutation that potentially make it became cancer. The scores were 3,00+0,00 for the lowest tumor weight and volume and 4,00+0,00 for the highest one. The IHC staining showed the tumor tissues has equal average scores for the vascularization (CD31) and apoptotic (caspase-3) scores. The CD31 IHC scores was 3,83+0,98 for non-localized brown color of DAB on the lowest tumor weight and volume. The CD31 IHC scores was 4,17+0,75 for non-localized brown color of DAB on the highest one. The caspase-3 IHC scores were 0,44+0,73 for localized and 3,33+1,58 for non-localized brown color of DAB on the lowest tumor weight and volume. The kaspase-3 IHC scores were 0,38+0,74 for localized and 3,38+0,92 for non-localized brown color of DAB on the highest one. These mean that the dose may decrease relatively the breast tumor weight and volume of the C3H mice.