Aplikasi probiotik, prebiotik, dan sinbiotik melalui pakan pada udang vaname Litopenaeus vannamei yang diinfeksi bakteri Vibrio harveyi

Abstract

Luminous vibriosis caused by Vibrio harveyi were commonly treated with antibiotic, but recently the use of antibiotics is restricted because it induces antibiotic-resistant bacteria and leave residue in shrimp’s body. An alternative solution that can be done to treated luminous vibriosis is by using applications of probiotic, prebiotic, and synbiotic. The aim of this research was to examine the effect of probiotic, prebiotic, and synbiotic on the survival rate and growth of pacific white shrimp against V. harveyi infection. Feed as treatment was supplemented with probiotic 1%, prebiotic 2%, and probiotik 1% + prebiotic 2% (synbiotic). Shrimps feed without supplementation of probiotic and prebiotic was used as control treatment. Shrimp test inserted into the aquarium (60x30x35) cm as much as 15 pic with a density of 40 shrimps/ 40 liters/ aquarium and an average weight of 0.4 ± 0.1 grams. After 30 days of feeding treatment, the shrimp was challenged by immersion with V. harveyi solution containing 106 CFU/ml. Survival rate of shrimp was measured before and after challenge while growth and feed convertion were observed before challenge test. Research result showed that growth (5.89%), feed convertion (1.21), and survival rate (80%) before challenge of shrimp fed diet supplemented with synbiotic were better then shrimp fed diet supplemented with probiotic and prebiotic. After challenge, survival rate (83.33%) of shrimp fed diet supplemented with synbiotic was better then prebiotic (51.67%) and positive control (31.67%).