Suplementasi Arginin dan Guanidinoacetic Acid dalam Pakan terhadap Profil Hematologi Ayam Broiler Fase Finisher

Abstract

Penelitian ini bertujuan mengevaluasi pengaruh suplementasi arginin dan guanidinoacetic acid (GAA) terhadap profil hematologi ayam broiler fase finisher. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan 6 perlakuan dan 5 ulangan. Perlakuan pakan terdiri atas P1 (kontrol positif), P2 (kontrol negatif), P3 = P2 + GAA 600 g MT-1, P4 = P2 + arginin 462 g MT-1 (rasio Arg/Lys 111%), P5 = P2 + arginin 600 g MT-1 (rasio Arg/Lys 112%), dan P6 = P2 + arginin 900 g MT-1 (rasio Arg/Lys 115%). Peubah yang diamati meliputi eritrosit, leukosit, hemoglobin, hematokrit, dan diferensiasi leukosit (limfosit, heterofil, eosinofil, monosit, basofil, dan rasio H:L). Hasil penelitian menunjukkan bahwa suplementasi arginin dan GAA berpengaruh nyata (P<0,05) terhadap jumlah eritrosit, hemoglobin, leukosit, eosinofil, dan monosit. Suplementasi arginin 462 g MT-1 dengan rasio Arg/Lys 111% meningkatkan jumlah eritrosit dan hemoglobin serta menurunkan jumlah leukosit dan persentase eosinofil dibandingkan kontrol negatif. Rasio Arg/Lys hingga 115% menurunkan jumlah eritrosit hingga berada di bawah kisaran fisiologis normal. Kesimpulan penelitian ini adalah suplementasi arginin 462 g MT-1 dengan rasio Arg/Lys 111% menghasilkan profil hematologi terbaik pada ayam broiler fase finisher.

This study aimed to evaluate the effects of arginine and guanidinoacetic acid (GAA) supplementation on the hematological profile of finisher broilers. A completely randomized design with 6 treatments and 5 replications was used. Dietary treatments consisted of P1 (positive control), P2 (negative control), P3 = P2 + 600 g MT-1 GAA, P4 = P2 + 462 g MT-1 arginine (Arg/Lys ratio 111%), P5 = P2 + 600 g MT-1 arginine (Arg/Lys ratio 112%), and P6 = P2 + 900 g MT-1 arginine (Arg/Lys ratio 115%). Variables observed included blood profile and leukocyte differential counts. Arginine and GAA supplementation significantly affected (P<0.05) erythrocyte count, hemoglobin concentration, leukocyte count, eosinophils, and monocytes. Arginine supplementation at 462 g MT-1 with an Arg/Lys ratio of 111% increased erythrocyte count and hemoglobin concentration while reducing leukocyte count and eosinophil percentage compared with the negative control. An Arg/Lys ratio of 115% reduced erythrocyte count below the normal physiological range. The conclusion of this study is that arginine supplementation at 462 g MT-1 with an Arg/Lys ratio of 111% produced the best hematological profile in finisher broilers.