ANALISIS IN VITRO PEMANFAATAN AMPAS TEH HIJAU DAN TEH HITAM SEBAGAI PAKAN ALTERNATIF UNTUK RUMINANSIA

Abstract

Penelitian ini bertujuan menganalisis fermentabilitas rumen dan kecernaan nutrien yang diberi pakan alternatif ampas teh hijau dan teh hitam, serta menentukan level substitusi ampas teh terhadap jerami yang optimal. Peubah yang diamati meliputi pH rumen, amonia atau NH3, dan Volatille Fatty Acid (VFA) serta nilai kecernaan bahan kering (KcBK) dan kecernaan bahan organik (KcBO). Desain penelitian menggunakan Rancangan Acak Kelompok (RAK) dengan pengelompokkan berdasarkan cairan rumen domba dan 5 perlakuan. Perlakuan terdiri dari Perlakuan 0 (kontrol) = Jerami padi 100%, Perlakuan 1 = Jerami padi 75% + ampas teh hijau 25%, Perlakuan 2 = Jerami padi 50% + ampas teh hijau 50%, Perlakuan 3 = Jerami padi 75% + ampas teh hitam 25%, Perlakuan 4 = Jerami padi 50% + ampas teh hitam 50%. Analisis data menggunakan ANOVA dan uji lanjut Duncan. Perlakuan berpengaruh nyata (p<0,05) pada peubah fermentabilitas yaitu NH3 dan VFA, serta kecernaan bahan kering (KcBK) dan bahan organik (KcBO). Pemberian jerami padi 50% dan ampas teh hijau 50% pada analisis in vitro mampu membantu meningkatkan fermentabilitas dan kecernaan nutrien pada rumen.

This study aims to analyze rumen fermentability and nutrient digestibility when fed alternative feed of green tea and black tea residues, as well as to determine the optimal level of tea residue substitution for straw. The observed variables include rumen pH, ammonia or NH3, and Volatile Fatty Acid (VFA), as well as the dry matter digestibility (DMD) and organic matter digestibility (OMD) values. The research design used a randomized block design (RBD) with grouping based on rumen fluid and 5 treatments. The treatments consist of Treatment 0 (control) = 100% rice straw, Treatment 1 = 75% rice straw + 25% green tea residue, Treatment 2 = 50% rice straw + 50% green tea residue, Treatment 3 = 75% rice straw + 25% black tea residue, Treatment 4 = 50% rice straw + 50% black tea residue. Data analysis was conducted using ANOVA and Duncan's multiple range test. The treatment had a significant effect (p<0.05) on the fermentation variables, namely NH3 and VFA, as well as on dry matter digestibility (DMD) and organic matter digestibility (OMD). The provision of 50% rice straw and 50% green tea waste in in vitro analysis was able to help improve fermentation and nutrient digestibility in the rumen.