Efektivitas Suplementasi ZnO-NPs dalam Pakan terhadap Kadar Zinc, Superoksida Dismutase, dan Malondialdehida Daging Ayam Broiler

Abstract

Ayam broiler sering kali dihadapkan pada tantangan lingkungan berupa suhu tinggi yang memicu kondisi heat stress. Sebagai upaya pencegahan stres oksidatif, suplementasi mineral zinc (Zn) dalam pakan menjadi sangat krusial. Penelitian ini bertujuan untuk mengevaluasi efektivitas suplementasi ZnO-NPs dalam pakan terhadap kadar Zn, aktivitas superoksida dismutase (SOD), dan kadar malondialdehida (MDA) pada daging ayam broiler. Penelitian menggunakan 200 ekor ayam broiler strain Cobb dengan Rancangan Acak Lengkap (RAL) yang terdiri atas 4 perlakuan dan 5 ulangan. Perlakuan yang diberikan yaitu P0 = ransum kontrol; P1 = ransum kontrol + ZnO-NPs 50 ppm; P2 = ransum kontrol + ZnO-NPs 75 ppm; dan P3 = ransum kontrol + ZnO-NPs 100 ppm. Peubah yang diamati meliputi kadar Zn, aktivitas enzim SOD, dan kadar MDA daging dada ayam umur 35 hari. Hasil penelitian menunjukkan bahwa suplementasi ZnO-NPs berpengaruh sangat berbeda nyata (P<0,01) terhadap kadar Zn, SOD, dan MDA. Kadar Zn tertinggi diperoleh pada P3 dengan dosis 100 ppm (36,605 ppm). Aktivitas SOD tertinggi terdapat pada P1 dengan dosis 50 ppm (13,527 U mg -1 ), sedangkan kadar MDA terendah terdapat pada P3 dengan dosis 100 ppm (1,693 mg kg -1 ). Simpulan penelitian ini menunjukkan suplementasi 50 ppm ZnO-NPs mampu meningkatkan kadar zinc dan aktivitas enzim superoksida dismutase daging dada ayam broiler. Pada suplementasi 100 ppm ZnO-NPs, kadar malondialdehida dalam daging dada ayam broiler tidak berbeda jauh dengan perlakuan kontrol.

Broiler chickens are frequently exposed to environmental challenges in the form of high temperatures that trigger heat stress conditions. To prevent oxidative stress, mineral zinc (Zn) supplementation in feed becomes crucial. This study aimed to evaluate the effectiveness of ZnO-NPs supplementation in feed on Zn levels, superoxide dismutase (SOD) activity, and malondialdehyde (MDA) levels in broiler chicken meat. The study utilized 200 Cobb strain broiler chickens in a Completely Randomized Design (CRD) consisting of 4 treatments and 5 replicates, namely P0 = control ration, P1 = control ration + 50 ppm ZnO-NPs, P2 = control ration + 75 ppm ZnO-NPs, and P3 = control ration + 100 ppm ZnO-NPs. The observed variables included meat Zn levels, SOD enzyme activity, and MDA levels in the breast meat of 35 days old broilers. The results showed that ZnO-NPs supplementation had a highly significant effect (P < 0.01) on Zn, SOD, and MDA levels. The highest Zn level was obtained in P3 at dose of 100 ppm (36.605 ppm), while the highest SOD activity was found in P1 at dose of 50 ppm (13.527 U mg?¹), and the lowest MDA level was found in P3 at dose of 100 ppm (1.693 mg kg?¹). This study concludes that supplementation of 50 ppm ZnO-NPs was able to increase zinc levels and superoxide dismutase enzyme activity in broiler chicken breast meat. At 100 ppm ZnO-NPs supplementation, the malondialdehyde levels in broiler chicken breast meat were not significantly different from the control treatment.