Profil Lipid dan Hematologi Darah Tikus Putih Hiperkolesterolemia yang diberi Kitosan Asal Maggot BSF (Black Soldier Fly)

Abstract

Penelitian ini bertujuan menganalisis profil lipid dan hematologi darah tikus putih hiperkolesterolemia yang diberi kitosan maggot BSF, serta mengevaluasi dosis pemberian kitosan yang optimal pada tikus putih hiperkolesterolemia. Penelitian ini menggunakan 50 ekor tikus putih jantan Sprague Dawley, terdiri atas 10 ekor tikus sehat dan 40 ekor tikus yang telah diinduksi hiperkolesterolemia menggunakan pakan hiperkolesterol dan propiltiourasil (PTU). Rancangan yang digunakan Rancangan Acak Lengkap (RAL) 5 perlakuan dan 10 ulangan. Perlakuan terdiri atas P0(Kontrol Sehat)= Pakan normal; P1(Kontrol Negatif)= Pakan hiperkolesterol + PTU 10 mg kg?¹ BB; P2(Kontrol Positif)= Pakan hiperkolesterol + obat atorvastatin 10 mg kg?¹ BB; P3= Pakan hiperkolesterol + kitosan maggot BSF 200 mg kg?¹ BB; P4= Pakan hiperkolesterol + kitosan maggot BSF 400 mg kg?¹ BB. Peubah yang diamati meliputi bobot badan (BB), pertambahan bobot badan (PBB), konsumsi pakan, profil lipid (kolesterol, trigliserida, LDL, HDL), hematologi darah, dan diferensiasi leukosit. Analisis data dengan analisis ragam dan uji lanjut Tukey HSD. Hasil menunjukkan bahwa pemberian kitosan tidak berpengaruh nyata (p>0,05) terhadap BB, PBB, hematologi darah, dan diferensiasi leukosit; Pemberian kitosan berpengaruh nyata (p<0,05) menurunkan kadar kolesterol, trigliserida, dan LDL. Pemberian kitosan dosis 400 mg kg?¹ BB lebih optimal menurunkan kadar kolesterol, trigliserida, LDL, serta tidak memengaruhi gambaran hematologi darah.

This study aims to analyze the blood lipid and hematological profiles of hypercholesterolemic Sprague Dawley rats administered BSF maggot chitosan, as well as to evaluate the optimal dosage of chitosan for hypercholesterolemic Sprague Dawley rats. This study used 50 male Sprague Dawley white rats, consisting of 10 healthy rats and 40 rats that had been induced with hypercholesterolemia using a high cholesterol diet and propylthiouracil (PTU). A completely randomized design (CRD) with 5 treatments and 10 replicates was used. The treatments consisted of P0(Healthy Control)= normal diet; P1(Negative Control)= high cholesterol diet + PTU 10 mg kg?¹ BW; P2 (Positive Control)= high cholesterol diet + atorvastatin 10 mg kg?¹ BW; P3= high cholesterol diet + BSF maggot chitosan 200 mg kg?¹ BW; P4= hypercholesterol diet + BSF maggot chitosan 400 mg kg?¹ BW. The observed variables included Body Weight (BW), Weight Gain (WG), feed intake, lipid profile (cholesterol, triglycerides, LDL, HDL), blood hematology, and leukocyte differentiation. Data analysis was performed using analysis of variance (ANOVA) and Tukey’s HSD post-hoc test. Results showed that chitosan supplementation had no significant effect (p>0.05) on BW, BWG, blood hematology, and leukocyte differentiation; chitosan supplementation significantly reduced (p<0.05) cholesterol, triglyceride, and LDL levels. Administration of chitosan at a dose of 400 mg kg?¹ body weight was more effective in reducing cholesterol, triglyceride, and LDL levels, and does not affect blood hematology parameters.