Suplementasi Probiotik dan Paraprobiotik Bacillus cereus BR2 untuk pencegahan Motile Aeromonads Septicemia pada Ikan Mas

Abstract

Ikan mas memiliki jumlah produksi tinggi di Indonesia. Selama melakukan kegiatan budidaya ikan mas tidak terlepas dari serangan penyakit bahkan hingga mengakibatkan kematian salah satunya yaitu MAS (Motile Aeromonads Septicemia). Suplementasi probiotik dan paraprobiotik Bacillus cereus BR2 melalui pakan dapat menjadi alternatif dari penggunaan antibiotik yang berlebihan dalam lingkungan budidaya. Penelitian ini bertujuan menguji efektivitas suplementasi probiotik dan paraprobiotik B. cereus BR2 melalui pakan terhadap kinerja pertumbuhan dan respon imun sebagai pencegahan penyakit MAS (Motile Aeromonads Septicemia). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 pengulangan yaitu K+ (Kontrol positif), K- (Kontrol negatif), A (Probiotik 1,0 x 108 CFU mL-1), B (Paraprobiotik 1,0 x 108 CFU mL-1). Ikan uji memiliki bobot berukuran rata-rata 6,11±0.05 gram dan dipelihara selama 30 hari dengan pemberian pakan sesuai perlakuan masing-masing. Setelah masa pemeliharaan ikan di uji tantang selama 7 hari dengan Aeromonas hydrophila 106 CFU mL-1, sebanyak 0,1 mL disuntikkan ke setiap ikan. Hasil penelitian menunjukkan bahwa suplementasi probiotik dan paraprobiotik B. cereus BR2 mampu meningkatkan kinerja pertumbuhan dan respon imun sebagai pencegahan penyakit MAS (Motile Aeromonads Septicemia), dengan perlakuan B (paraprobiotik 108 CFU mL-1) sebagai hasil yang terbaik yang berbeda nyata (P<0,05) dengan kontrol.

Common carp (Cyprinus carpio) is one of the major freshwater aquaculture commodities with high production in Indonesia. However, common carp culture is frequently threatened by disease outbreaks, particularly Motile Aeromonads Septicemia (MAS), which can cause significant mortality. Dietary supplementation with probiotic and paraprobiotic Bacillus cereus BR2 has the potential to serve as an alternative to the excessive use of antibiotics in aquaculture systems. This study aimed to evaluate the effectiveness of dietary supplementation with probiotic and paraprobiotic B. cereus BR2 on growth performance and immune response for the prevention of Motile Aeromonads Septicemia (MAS) in common carp. The experiment employed a Completely Randomized Design (CRD) consisting of four treatments with three replicates: K+ (positive control), K- (negative control), A (Probiotic 1,0 x 108 CFU mL?¹), and B (Paraprobiotic 1,0 x 108 CFU mL?¹). xperimental fish with an average body weight of 6.11 ± 0.05 g were reared for 30 days and fed diets according to each treatment. Following the feeding trial, fish were challenged with Aeromonas hydrophila at a concentration of 106 CFU mL?¹ for 7 days by intramuscular injection of 0.1 mL per fish. The results demonstrated that dietary supplementation with probiotic and paraprobiotic B. cereus BR2 significantly improved growth performance and immune response, thereby enhancing resistance against Motile Aeromonads Septicemia (MAS). Among the treatments, paraprobiotic supplementation at 108 CFU mL?¹ showing the best result and significantly different (P<0.05) compared to the control.