dc.contributor.advisor | Nuryati, Sri | |
dc.contributor.author | Widiyani, Eka Oktavi | |
dc.date.accessioned | 2022-08-22T05:58:17Z | |
dc.date.available | 2022-08-22T05:58:17Z | |
dc.date.issued | 2022-08-22 | |
dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/113878 | |
dc.description.abstract | Pathogen that causes tilapia to develop Streptococcosis is pathogenic
bacterium Streptococcus agalactiae. This study aimed to test the polyvalent DNA
vaccine on tilapia (Oreochromis niloticus) infected with Streptococcosis which
causes mass mortality in tilapia. The test fish in this study had a weight range of
6.73±2.25 grams with length of 7.45±2.54 cm. Streptococcus agalactiae bacterial
isolates were obtained from the Aquatic Organism Health Laboratory. Tilapia was
vaccinated using Streptococcus agalactiae vaccine, Aeromonas hydrophila
vaccine, polyvalent vaccine dose 2.5 ng µL-1
, dose 5 ng µL-1
and dose 10 ng µL-1
and negative positive controls were only injected with PBS (Phosphate Buffered
Saline) solution. Tilapia was then challenged using Streptococcus agalactiae
bacteria with a density of 106 CFU mL
-1
for 0.1 mL in 14 days. Parameters were
performed before vaccination, after vaccination and after the trial. The test
parameters observed were total erythrocytes, total leukocytes, hemoglobin levels,
hematocrit levels, differential leukocytes, phagocytic activity, Relative Percent
Survival (RPS), growth rate and antibody titer. Vaccination with polyvalent DNA
vaccine was able to increase non-specific immune responses (total leukocytes,
lymphocyte percentage, and phagocytic activity) and specific in the form of
increasing antibody titers from tilapia. The survival of vaccinated tilapia was better
than the control. Based on research data, the highest RPS value was found in the 10
dose polyvalent vaccine treatment. The 10 dose polyvalent vaccine RPS value was
75.56%. | id |
dc.description.abstract | Patogen yang menyebabkan ikan nila terserang penyakit Streptococcosis adalah bakteri patogen Streptococcus agalactiae. Penelitian ini bertujuan menguji vaksin DNA polivalen pada ikan nila (Oreochromis niloticus) yang terinfeksi penyakit Streptococcosis penyebab kematian massal pada ikan nila. Ikan uji dalam penelitian ini memiliki kisaran bobot 6,73±2,25 g dengan panjang 7,45±2,54 cm. Isolat bakteri Streptococcus agalactiae diperoleh dari Laboratorium Kesehatan Organisme Akuatik. Ikan nila divaksin dengan menggunakan vaksin FKC, VDP dosis 2,5 ng µL-1, VDP dosis 5 ng µL-1, VDP dosis 10 ng µL-1. serta kontrol negatif dan positif hanya disuntik larutan PBS (Phosphate Buffered Saline). Ikan nila selanjutnya dilakukan uji tantang dengan menggunakan bakteri Streptococcus agalactiae dengan kepadatan 106 CFU mL-1 sebanyak 0,1 mL selama 14 hari. Pengamatan parameter dilakukan sebelum vaksinasi, setelah vaksinasi dan setelah uji tantang. Parameter uji yang diamati yaitu, total eritrosit, total leukosit, kadar hemoglobin, kadar hematokrit, diferensial leukosit, aktivitas fagositik, Relative Percent Survival (RPS), laju pertumbuhan dan titer antibodi. Vaksinasi dengan vaksin DNA polivalen mampu meningkatkan respons imunitas non spesifik (total leukosit, persentase limfosit, dan aktivitas fagositik) dan spesifik berupa peningkatan titer antibodi dari ikan nila. Kelangsungan hidup ikan nila yang divaksinasi lebih baik dibandingkan kontrol. Berdasarkan data penelitian, nilai RPS tertinggi terdapat pada perlakuan vaksin polivalen dosis 10. Nilai RPS vaksin polivalen dosis 10 yaitu 75,56%. | id |
dc.language.iso | id | id |
dc.publisher | IPB University | id |
dc.title | Uji Vaksin DNA Polivalen pada Ikan Nila
(Oreochromis niloticus) untuk Mencegah Streptococcosis. | id |
dc.title.alternative | Polyvalent Vaccine DNA on Tilapia (Oreochromis niloticus) to Prevent Streptococcosis | id |
dc.type | Undergraduate Thesis | id |
dc.subject.keyword | DNA vaccine, fish, pathogen, polyvalent, Streptococcus agalactiae | id |