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      Aplikasi Probiotik Lactobacillus plantarum dan Bacillus cereus untuk Pencegahan Vibriosis pada Udang Vaname Litopenaeus vannamei

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      Date
      2022-02
      Author
      Kevin, Immanuel Surya
      Widanarni
      Yuhana, Munti
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      Abstract
      Penggunaan antibiotik untuk pengendalian vibriosis pada udang vaname menimbulkan beberapa permasalahan seperti resistansi bakteri patogen serta residu antibiotik pada produk akuakultur dan lingkungan perairan. Probiotik diharapkan dapat menjadi alternatif lain yang ramah lingkungan. Penelitian ini bertujuan untuk menguji pengaruh pemberian probiotik L. plantarum dan B. cereus serta gabungan keduanya terhadap respons imun dan resistansi udang vaname yang diinfeksi V. parahaemolyticus. Udang vaname yang digunakan memiliki bobot awal 0.55+0.01 g, dipelihara selama 40 hari dengan perlakuan probiotik kemudian diuji tantang dengan V. parahaemolyticus dan diamati respons imun dan resistansinya selama tujuh hari. Perlakuan yang diberikan meliputi kontrol positif (K+), kontrol negatif (K-), probiotik L. plantarum (Lp), probiotik B. cereus (Bc), dan gabungan probiotik (Lp+Bc). Hasil penelitian menunjukkan bahwa kinerja pertumbuhan, respons imun dan resistansi udang vaname yang diberikan probiotik lebih baik dibandingkan kontrol. Respons imun terbaik pada sebelum maupun setelah uji tantang dengan V. parahaemolyticus terdapat pada perlakuan Lp+Bc. Pemberian probiotik L. plantarum dan B. cereus serta gabungan keduanya mampu meningkatkan kinerja pertumbuhan, respons imun dan resistansi udang vaname terhadap infeksi V. parahaemolyticus, dengan nilai respons imun terbaik pada perlakuan gabungan kedua probiotik.
       
      The use of antibiotics to control vibriosis in white shrimp raises several problems such as resistance to pathogenic bacteria and antibiotic residues in aquaculture products and the aquatic environment. Probiotics are expected to be another environmentally friendly alternative. This study aimed to examine the effect of giving probiotics L. plantarum and B. cereus and their combination on the immune response and resistance of white shrimp infected with V. parahaemolyticus. The white shrimp used had an initial weight of 0.55+0.01 g, reared for 40 days with probiotic treatment and then challenged with V. parahaemolyticus and observed immune response and resistance for seven days. The treatments included positive control (K+), negative control (K-), L. plantarum probiotic (Lp), B. cereus probiotic (Bc), and a combination of probiotics (Lp+Bc). The results showed that the performance growth, immune responses, and resistance of white shrimp given probiotics was better than the control. The best immune response before and after the challenge test with V. parahaemolyticus was found in Lp+Bc treatment. The administration of probiotics L. plantarum and B. cereus and their combination was able to increase growth performance, immune response, and resistance of white shrimp against V. parahaemolyticus, with the best immune response value in combined treatment of two probiotics.
       
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      http://repository.ipb.ac.id/handle/123456789/111223
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      • UT - Aquaculture [2189]

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      Indonesia DSpace Group 
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