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      Uji Efektivitas Madu dari Empat Spesies Lebah Berbeda sebagai Agen Antibakteri.

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      Date
      2022
      Author
      Devi, Andhika Rahesta
      Astuti, Rika Indri
      Raffiudin, Rika
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      Abstract
      Banyak penyakit yang diakibatkan oleh bakteri. Pengobatan infeksi dapat dilakukan dengan memberikan antibakteri pada luka. Salah satu antibakteri alami adalah madu. Tujuan dari penelitian ini adalah menganalisis efektivitas madu sebagai agen antibakteri. Bakteri target yang digunakan adalah Escherichia coli ATCC 8739, Staphylococcus aureus ATCC 6538 dan Pseudomonas aeruginosa ATCC 15442. Uji antibakteri terdiri dari 4 tahap yaitu uji disk, uji viabilitas, uji pH dan uji proksimat. Hasil uji antimikroba menunjukkan bahwa madu TG1_PND yang berasal dari Stingless Bee memiliki aktivitas antibakteri tertinggi dibanding madu lainnya. Hasil uji viabilitas menunjukkan ketiga bakteri mengalami penghambatan pertumbuhan terbaik dalam konsentrasi madu 100%. Adapun kandungan pH madu diuji melalui pengukuran menggunakan pH meter dan diketahui bahwa madu dengan kode TG1_PND memiliki pH terendah yaitu 2,7. Kandungan air pada madu dapat memengaruhi kualitas madu dan pertumbuhan mikroba.
       
      Many diseases are caused by bacteria. Treatment of infection can be done by giving antibacterial to the wound. One natural antibacterial is honey.The purpose of this study was to analyze the potential of honey as an antibacterial. The target bacteria used were Escherichia coli ATCC 8739, Staphylococcus aureus ATCC 6538 and Pseudomonas aeruginosa ATCC 15442. The test consisted of 4 stages, namely disk test, viability test, pH test and proximate test. The antibacterial test results showed that TG1_PND honey from Stingless Bee had the highest antibacterial activity compared to other honeys. The results of the viability test showed that the three bacteria experienced the best growth inhibition in 100% honey concentration. The pH content of honey was tested by measuring using a pH meter and it was found that honey with the code TG1_PND had the lowest pH of 2.7. Water content in honey can affect honey quality and microbial growth.
       
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      http://repository.ipb.ac.id/handle/123456789/110579
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