Toleransi Tanaman Siratro (Macroptilium atropurpureum) terhadap Kondisi Masam melalui Teknik Kultur Jaringan

Abstract

Siratro merupakan leguminosa yang dapat tumbuh pada daerah tropis dan subtropis, serta tahan terhadap kekeringan. Penelitian ini bertujuan untuk mengetahui tingkat toleransi tanaman siratro (Macroptilium atropurpureum) terhadap kondisi masam dengan level yang berbeda melalui teknik kultur jaringan. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan 6 perlakuan penambahan AlCl3 dengan ulangan tidak sama (P0 (0 ppm AlCl3), P1 (100 ppm AlCl3), P2 (200 ppm AlCl3), P3 (300 ppm AlCl3), P4 (400 ppm AlCl3), dan P5 (500 ppm AlCl3)). Data dianalisis menggunakan analisis deskriptif, analisis korelasi, dan analisis varian (ANOVA), jika ada signifikasi akan diuji lanjut menggunakan uji Duncan. Peubah yang diamati adalah pertambahan tinggi tanaman, persentase kerontokan daun, penyusutan media, pertambahan bobot tanaman, warna daun, jumlah daun, perubahan pH media, dan persentase kontaminasi. Hasil analisis ragam terhadap parameter pertambahan tinggi tanaman, persentase kerontokan daun, penyusutan media, dan pertambahan bobot tanaman berbeda nyata (P<0,05). Tingkat toleransi tanaman siratro (Macroptilium atropurpureum) pada pemberian aluminium sampai level 100 ppm (pH 4,91). Kata kunci : aluminium, asam, pertumbuhan, pH, siratro, tanaman, toleransi ABSTRACT LISNI TASYA. Tolerance of Siratro Plants (Macroptilium atropurpureum) to Acidic Soil Conditions through Tissue Culture Technique. Supervised by PANCA DEWI MANU HARA KARTI and DIDID DIAPARI. Siratro is a legume that can grow in tropical and subtropical areas, and is resistant of drought. The experiment was aimed to determine the tolerance level of siratro (Macroptilium atropurpureum) plants to acidic soil condition with different levels through tissue culture techniques. This study was used a complete randomized design with 6 treatments with the addition of AlCl3 (P0 (0 ppm AlCl3), P1 (100 ppm AlCl3), P2 (200 ppm AlCl3), P3 (300 ppm AlCl3), P4 (400 ppm AlCl3), dan P5 (500 ppm AlCl3)) with different number of replications. Data were analyzed using descriptive analysis, correlation analysis, and analysis of variance (ANOVA), if there was significant difference data were further analyzed using using Duncan test. The variables observed were plant height increasing, percentage of leaf shedding, media depreciation, increased of plant weight, leaf color, number of leaf, acidy media changes, and percentage of contamination. This treatment significantly (P<0,05) influences plant height increasing, percentage of leaf shedding, media depreciation, and increased of plant weight. The tolerance level of siratro (Macroptilium atropurpureum) on giving aluminum level until 100 ppm (pH 4,91). Keywords : acids, aluminum, growth, pH, plants, siratro, tolerance

Siratro merupakan leguminosa yang dapat tumbuh pada daerah tropis dan subtropis, serta tahan terhadap kekeringan. Penelitian ini bertujuan untuk mengetahui tingkat toleransi tanaman siratro (Macroptilium atropurpureum) terhadap kondisi masam dengan level yang berbeda melalui teknik kultur jaringan. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan 6 perlakuan penambahan AlCl3 dengan ulangan tidak sama (P0 (0 ppm AlCl3), P1 (100 ppm AlCl3), P2 (200 ppm AlCl3), P3 (300 ppm AlCl3), P4 (400 ppm AlCl3), dan P5 (500 ppm AlCl3)). Data dianalisis menggunakan analisis deskriptif, analisis korelasi, dan analisis varian (ANOVA), jika ada signifikasi akan diuji lanjut menggunakan uji Duncan. Peubah yang diamati adalah pertambahan tinggi tanaman, persentase kerontokan daun, penyusutan media, pertambahan bobot tanaman, warna daun, jumlah daun, perubahan pH media, dan persentase kontaminasi. Hasil analisis ragam terhadap parameter pertambahan tinggi tanaman, persentase kerontokan daun, penyusutan media, dan pertambahan bobot tanaman berbeda nyata (P<0,05). Tingkat toleransi tanaman siratro (Macroptilium atropurpureum) pada pemberian aluminium sampai level 100 ppm (pH 4,91). Kata kunci : aluminium, asam, pertumbuhan, pH, siratro, tanaman, toleransi ABSTRACT LISNI TASYA. Tolerance of Siratro Plants (Macroptilium atropurpureum) to Acidic Soil Conditions through Tissue Culture Technique. Supervised by PANCA DEWI MANU HARA KARTI and DIDID DIAPARI. Siratro is a legume that can grow in tropical and subtropical areas, and is resistant of drought. The experiment was aimed to determine the tolerance level of siratro (Macroptilium atropurpureum) plants to acidic soil condition with different levels through tissue culture techniques. This study was used a complete randomized design with 6 treatments with the addition of AlCl3 (P0 (0 ppm AlCl3), P1 (100 ppm AlCl3), P2 (200 ppm AlCl3), P3 (300 ppm AlCl3), P4 (400 ppm AlCl3), dan P5 (500 ppm AlCl3)) with different number of replications. Data were analyzed using descriptive analysis, correlation analysis, and analysis of variance (ANOVA), if there was significant difference data were further analyzed using using Duncan test. The variables observed were plant height increasing, percentage of leaf shedding, media depreciation, increased of plant weight, leaf color, number of leaf, acidy media changes, and percentage of contamination. This treatment significantly (P<0,05) influences plant height increasing, percentage of leaf shedding, media depreciation, and increased of plant weight. The tolerance level of siratro (Macroptilium atropurpureum) on giving aluminum level until 100 ppm (pH 4,91). Keywords : acids, aluminum, growth, pH, plants, siratro, tolerance