Deteksi Kualitatif Near Infrared Reflectance Spectroscopy (NIRS) Berdasarkan Profil Asam Lemak Susu Sapi Perah

Abstract

Penelitian ini bertujuan membuat model kalibrasi menggunakan Near Infrared Reflectance Spectroscopy (NIRS) dari susu sapi perah dengan waktu pemerahan yang berbeda. Pengambilan sampel dilakukan di peternakan rakyat yang berlokasi di tiga tempat yaitu Pangalengan, Cibungbulang, dan Kebon Pedes Jawa Barat. Komponen sampel susu dianalisis menggunakan lactoscan dan identifikasi profil asam lemaknya menggunakan gas chromatography (GC). Sampel susu dianalisis secara rapid menggunakan NIRS. Hasil analisis komponen susu dan asam lemak diolah secara statistik dengan uji normalitas dan uji-t pada SPSS ver. 25. Hasil berbeda nyata didapatkan ada tiga parameter dari susu pemerahan pagi dan sore (P<0,05) yaitu lemak susu, C8:0 dan C14:1. Hasil analisis pada NIRS menunjukkan susu pemerahan pagi dan sore memiliki spektrum serupa yang ditunjukkan dengan nilai Q-value yang rendah (0.0011231). Analisis secara kualitatif pada NIRS tidak dapat membedakan susu hasil pemerahan pagi dan sore hari.

Kata kunci: kualitas susu, NIRS, profil asam lemak, susu, waktu pemerahan.

The objective of this study was to create calibration model using Near Infrared Reflectance Spectroscopy (NIRS) from dairy milk with the differences milking time. Milk sampling were collected from three different farms location in Pangalengan, Cibungbulang, and Kebon Pedes West Java. The quality of the milk samples were analyzed using lactoscan and gas chromatography (GC) to identified the FA profiles of milk. Sample also analyzed rapidly using NIRS. The result of analyzed using lactoscan and GC were stastistically tested using normality test and t-test carried out SPSS ver. 25. Analysis on GC and lactoscan it were found three parameters that significantly different (P<0.05) between morning and afternoon milking time, namely milk fat, C8:0 and C14:1. However, the result using NIRS indicate that all parameters have similar spectrum between morning and afternoon milking that marked by low Q-value (0.0011231). It is concluded that NIRS spectrum can not differentiate milk from morning and afternoon milking qualitatively.

Keywords: FA profiles, milk, milking time, NIRS, quality of milk.