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Title: | Analisis Proksimat dan Aktivitas Penghambatan Madu Hutan, Rambutan dan Kelengkeng Terhadap Sel Kanker MCF-7 |
Other Titles: | Proximate Analysis and Inhibition Activities of Forest Honey, Rambutan Honey and Longan Honey on Cancer Cells MCF-7 |
Authors: | Budiman, Cahyo Soenarno, Mochammad Sriduresta Riadi, Ahda Sabila |
Issue Date: | 2022 |
Abstract: | Kanker adalah penyakit akibat pertumbuhan sel-sel jaringan tubuh yang
abnormal. Penanganannya yang mahal dan berbahaya mendorong pencarian
pengobatan alternatif dengan sumber alam. Madu diketahui memiliki komposisi
senyawa yang bersifat antikanker. Penelitian ini bertujuan untuk mengkaji aktivitas
antikanker madu rambutan, kelengkeng, dan hutan dan kandungan proksimatnya.
Analisis proksimat terdiri atas analisis kadar air, abu, protein, lemak, energi total
dan karbohidrat. Hasil uji menunjukkan bahwa madu mengandung lemak total
(<0,02%) dan protein (0,04%) yang rendah. Selanjutnya, aktivitas antikanker diuji
menggunakan sel kanker payudara MCF-7 dengan metode MTT (3-(4,5-
dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide) dengan tiga kali
pengulangan. Madu menunjukkan aktivitas penghambatan yang sangat lemah
terhadap sel MCF-7. Madu rambutan memiliki aktivitas penghambatan tertinggi
(121,81±2,04 ppm) diikuti madu kelengkeng (199,32±1,60 ppm) dan madu hutan
(339,24±1,02 ppm). Kendati aktivitas penghambatannya yang lemah, madu
menjanjikan untuk dikembangkan lebih lanjut sebagai pengobatan antikanker
dengan mengisolasi senyawa yang bertanggung jawab untuk aktivitas antikanker. Cancer is a disease caused by abnormal growth of body tissue cells. Expensive and dangerous treatment encourage the search for alternative medicine with natural sources. Honey is known for its anticancer properties. This study aims to examine the anticancer activity of rambutan, longan, and forest honey and their proximate content. Proximate analysis consisted of analysis of water content, ash, protein, fat, total energy and carbohydrates. The proximate test results showed that honey contained very low total fat (<0.02%) and protein (0.04%). Furthermore, the anticancer activity of honey was tested using MCF-7 breast cancer cells with the MTT test (3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide) with three repetitions. Honey showed very weak inhibitory activity against MCF-7. Rambutan honey had the best inhibitory activity (121.81±2.04 ppm) followed by longan honey (199.32±1.60 ppm) and forest honey (339.24±1.02 ppm). The weak inhibition, nevertheless, promises the honey to be further developed as anticancer treatments by isolating the compounds responsible for the anticancer activity. |
URI: | http://repository.ipb.ac.id/handle/123456789/115449 |
Appears in Collections: | UT - Technology of Cattle Products |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Cover_D34170042_Ahda Sabila Riadi.pdf Restricted Access | Cover | 344.89 kB | Adobe PDF | View/Open |
D34170042_Ahda Sabila Riadi.pdf Restricted Access | Full text | 723.39 kB | Adobe PDF | View/Open |
Lampiran_D34170042_Ahda Sabila Riadi.pdf Restricted Access | Lampiran | 642.07 kB | Adobe PDF | View/Open |
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