The morphological of fetal lung of doe injected with PMSG hormon prior mating.

Abstract

PMSG hormone is one of the reproductive hormones that play a role in determining embryonic and fetal development during the pregnancy period. The report shows that PMSG hormone able to improve the parent's hormonal conditions during the pregnancy period by triggering an increase of follicles number and the corpus luteum. The information on the effect of PMSG hormone to the mother prior mating in order to improve hereditary quality in general is still lacking, particular to the fetal lung development. This study was designed to evaluate the morphological development of the lung fetal, which delivered by PMSG hormone induced doe. The evaluation of lung tissues was carried out on three fetal lungs, which were from control doe without PMSG induction (IN), PMSG induced doe (7,5 IU/kg BW/IP7,5), and PMSG induced doe in dose of (15 IU/kg BB /IP15). The lung tissue samples were processed by histological method and stained by Hematoxylin Eosin (HE) staining then measured its pleura thickness, bronchus diameter, and the diameter of alveolus. The analysis was done using a t-test on Microsoft Excel. The results of the study showed that anatomically the lungs were fully developed which showed a complete lobe formation. Histological image also showed that the development of the respiratory system of the alveolar duct, alveolar sac, and fetal alveol has developed larger in the IP fetus than the IN fetus. In conclusion, the injection of PMSG hormone in the mother goat can accelerate the development of parts of the lungs that can be seen in the tip of bronchial gland and the IP fetal lungs growing larger than the fetus IN.