dc.contributor.advisor | Suharsono | |
dc.contributor.advisor | Roberdi | |
dc.contributor.author | Borlay, Allen Johnny | |
dc.date.accessioned | 2017-08-07T03:58:47Z | |
dc.date.available | 2017-08-07T03:58:47Z | |
dc.date.issued | 2017 | |
dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/87665 | |
dc.description.abstract | Oil palm (Elaeis guineensis Jacq.) is an important economic crop species
due to the versatile applications of its crude and kernel oils. The necessity to
improve oil palm, which is the world’s most productive oil producing plant under
cultivation currently for high yield and oil quality to meet the growing global
demand especially in term of its healthy component, oleochemical industrial needs
and biodiesel utilization has become a major area of focus for oil palm breeders.
Marker assisted selection have played a crucial role in oil palm breeding programs
and single nucleotide polymorphism (SNP) maker is one of those technology that
has recently attracted breeders. High content of oleic acid (18:1) has proven to be
that needed component of oil palm and other vegetable oils that can greatly
contribute to oil quality improvement. Stearoyl Acyl-carrier-protein Desaturase
(SAD) is a key enzyme for oleic acid biosynthesis which plays an important role in
determining the composition of unsaturated fatty acids in oil palm.
In oil palm, SAD gene is located in chromosome 7, 8, and 9. This research
focused on chromosome 7 and 9. There are 3 exon region in each of these two
chromosomes. Specific primers were designed base on the 6 exon and neighboring
intron region. Genomic DNA from three oil palm sources were amplified using
these specific primers. We identified 9 SNP loci (4 in the exon and 5 in the intron)
in SAD gene fragment and developed 9 allele-specific single nucleotide amplified
polymorphism (SNAP) marker for oleic acid prediction. Four out of the nine SNAP
marker developed and tested on 25 oil palm accessions were polymorphic and
reasonably informative. The average expected and observed heterozygosities were
0.391 and 0.404, respectively. The mean polymorphism information content (PIC)
was 0.312. Our results showed that these SNAP markers will be useful if validated
in larger oil palm population in predicting oleic acid composition and genetic
variation in oil palm breeding programs based on SAD gene fragment.
This research account for the first specific study of SNAP marker development
based on SNPs loci in both intron and exon region of SAD gene sequence in
chromosomes 7 and 9 of oil palm. The result of our research will allow direct MAS
specifically for oleic acid content and generally for unsaturated fatty acid prediction
in oil palm at an early growth stage with less resources and time and also carried on
genetic diversity analysis based on SAD gene. In addition, this research result will
have set the pitch for further genetic diversity studies on the progeny of cross
between the species of oil palm (E.guineensis and E.oleifera). Initial evaluation of the
SNAP markers developed in this study is significantly beneficiary for oil palm
breeders. | id |
dc.language.iso | id | id |
dc.publisher | Bogor Agricultural University (IPB) | id |
dc.subject.ddc | Horticulture | id |
dc.subject.ddc | oil palm | id |
dc.subject.ddc | 2016 | id |
dc.subject.ddc | Infonesia | id |
dc.title | Development of Single Nucleotide Polymorphisms (SNPs) Marker based on SAD Gene in Oil Palm (Elaeis guineensis Jacq.) | id |
dc.type | Thesis | id |
dc.subject.keyword | Allele-specific | id |
dc.subject.keyword | expected heterozygosities | id |
dc.subject.keyword | observed heterozygosities | id |
dc.subject.keyword | oil quality | id |
dc.subject.keyword | SAD | id |
dc.subject.keyword | SNP | id |