Penapisan Deteksi PCR ACC Deaminase dan Analisis Produksi Auksin Rhizosfer Tanaman Padi Daerah Pesisir

Abstract

Salinity and drought stress results in the production of ethylene. Rhizosphere bacterial activity suppresses the production of ethylene through the activity of 1-aminocycopropane-1-carboxylate (ACC) deaminase. In this study, a sampel of rhizosphere bacteria from coastal rice plant area was tested. The method used was acc deaminase activity test performed on Dworkin - Foster (DF) media and PCR acdS gene using specific primers of ACC and a quantitative analysis of the production of auxin (IAA). Of 47 isolates obtained, 8 were positively have acdS activity. The positive result was indicated by the presence of 1080 bp amplicon. Quantitative analysis showed the highest yield of 10.6 ppm of auxin. The results prove that there are some bacteria originated from coastal rice plant area which have acc deaminase activity.