Inisiasi Rimpang Mikro Kaempferia parviflora Secara In Vitro dengan Penambahan BAP dan Sukrosa

Abstract

Kaempferia parviflora is a medicinal plant that contains secondary metabolites and efficacious for treating various diseases. Rapid multiplication of K. parviflora through its rhizome is hamprered by the long period needed to produce the rhizome. The objectives of this research were to obtain the best sucrose concentration and to determine the best BAP concentration in the in vitro micro rhizome initiation of K. parviflora. The experiment was arranged in a randomized complete block design with two factors and three replications. The first factor was BAP concentration, consisted of three levels i.e. 0, 2, and 4 mg L¯1. The second factor was sucrose concentration, consisted of four levels i.e. 0, 30, 60, and 90 g L¯1. Data obtained were analyzed by F-test, followed by DMRT (Duncan Multiple Range Test) at 5 % significant level. Sucrose concentration significantly affected the number of shoots and plantlets fresh weight. The results showed that K. parviflora could form micro rhizome in vitro, as indicated by the similar anatomical structure compared to the rhizome produced in vivo. K. parviflora cultured in MS medium cointaining 90 g L¯1 sucrose without BAP had the highest number of micro rhizome at 8 WAC.