Kadar Total Senyawa Fenolik, Flavonoid, dan Aktivitas Antioksidan Ekstrak Air dan Ekstrak Metanol Daun Singkong (Manihot esculenta Crantz)

Abstract

Manihot esculenta Crantz or cassava is a plant that has been known, but their leaves utilization is still limited as animal feed additives. Cassava leaves contain mineral such as Fe, Zn, Mn, Cu, Mg, Ca, P, K, S, contain crude protein, β- carotene and have an active compound of flavonoids, phenolic, and contain chlorophyll which is a natural antioxidant. The purpose of this study were to determine the levels of total phenolics, flavonoids, and antioxidant activity of the water extracts and methanol extracts of simplicia and boiled cassava leaves in vitro. Cassava leaves extraction was done by maceration method to obtain the methanol extract and infundation method to get the water extract. Based on phytochemical test, cassava leaves contain alkaloids, flavonoids, phenolic, tannins, and saponins. The highest levels of total phenolics and flavonoids contained in the methanol extract of simplicia, 30.57 mg GAE/g and 881.33 mg RE/g. Cassava leaves extract has potential as an antioxidant with the highest inhibition of DPPH radicals produced by the methanol extract of simplicia which have IC50 values of 92.10 mg/L and followed by methanol extract of boiled leaves, water extract of simplicia, and water extract of boiled leaves