Aktivitas Penghambatan Ekstrak Sirih Merah Piper crocatum terhadap Pembentukan Malondialdehida (MDA) dan Enzim Tirosinase

Abstract

Piper crocatum leave bark is a medicinal plant. It contains antioxidant compounds. The objectives of this research was to determine the antioxidant activity and inhibitory activity to tyrosinase of P. crocatum extract. Extraction process of Piper crocatum was conducted separately by using the solvent ethanol 70% and n-hexane. Determination of antioxidant activity was conducted with Thiobarbituric acid method (TBA) and for tyrosinase inhibitory activity using microplate reader. The results showed that the ethanol extract at concentration of 200 ppm higher (α>0.05), inhibitory effects than vitamin E on the formation malondialdehida. n-hexane extract at concentration of 200 ppm higher (α>0.05). Tyrosinase inhibition activity test showed the ethanol extract has IC50 1655 ppm, n-hexane extract has IC50 3090.56 ppm. Ethanol extract has inhibitory MDA and enzyme tyrosinase than n-hexane extract.