Pemanfaatan Konsorsia Oleaginous untuk Produksi Biodiesel Menggunakan Substrat Palm Oil Mill Effluent (POME)

Abstract

Oleaginous accumulate lipid in the bodies and able to use cellulose in POME (Palm Oil Mill Effluent) as sole carbon source for lipid accumulation. Enzyme produce by microorganism is able to hydrolyze cellulose into fermentable sugar. Hydrolytic fungi used in study ATH 2147, S-30, S-50, and oleaginous yeast selected was Y34. The purpose of this study is to determine the hydrolysis activities and the most optimal lipid accumulation biodiesel production and determine the effect of temperature, microbial inoculants, and the incubation time on hydrolysis and lipid accumulation. Hydrolysis and lipid accumulation process at 30oC and 50oC. Based on the analysis, it was found that consortia of ATH 2147+Y34 produce highest cellulose enzyme activity at a temperature of 50oC at day 4 and the highest lipid accumulation at 30oC at pH 4.3 after 6 days cultivation. Fatty acid methyl ester composition was dominated by methyl butanoate, palmitic acid, and stearic acid. These consortia could be used for second-generation biofuel research.