Study of Supplementation of Dietary Probiotic, Prebiotic, and Synbiotic to Precaution Vibriosis in Polkadot Grouper (Cromileptes altivelis).
Abstract
Vibriosis was a major bacterial diseases in grouper fingerlings and very harmful. One of the diseases that often attack the humpback grouper fish was bacterium V. alginolyticus. V. alginolyticus attack on marine fish could reach up to 100% mortality. Application of probiotics, prebiotics and synbiotic through feed was expected to be one of the ways to boost the immune system of the fish. This study aimed to assess the effect of probiotics, prebiotics, and synbiotic on humpback grouper feed for vibriosis infection prevention through observation of immune response, survival and growth performance. The research covers the preparation stage and the media container maintenance, preparation of probiotic, preparation of prebiotic, feed preparation, testing probiotic, prebiotic, and synbiotic through the feed. There were five tests in the commission of the feed, feeding without probiotic, prebiotic, and synbiotic and PBS injected, feeding without probiotic, prebiotic, and synbiotic and infected V. alginolyticus, feeding of 1% with probiotic and infected V. alginolyticus, feeding of 2% prebiotic and infected V. alginolyticus, feeding with probiotics 1% and prebiotics 2 % and infected V. alginolyticus. Challenge test was injecting V. alginolyticus intramuscularly. Testing the performance of growth at the end of treatment probiotic, prebiotic, and synbiotic by measuring the daily growth rate and feed conversion ratio. Immune response testing was also conducted by measuring the total erythrocytes, total leukocytes, hemoglobin, hematocrit, respiratory burst activity, phagocytic activity and total bacteria. The characterization of probiotic was conducted by Cowan. The result showed that the bacterium was Bacillus sp. The result of oligosaccharide extraction (TPT 5%) used in this study consist of 1.115% inulin; 1.015% FOS and 1.488% GOS. Feeding with the addition of probiotic, prebiotic, and synbiotic for thirty days can increase the survival rate and growth performance (DGR and FCR) and resistance to disease. Overall immune response results obtained in this study showed significant improvement between the administration of probiotics, prebiotics, and synbiotic to control.
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- MT - Fisheries [3011]