Identifikasi Bakteri Endofit Isolat Biogen CC-E76 dengan Teknik 16S rRNA

Abstract

Endophytic bacteria is bacteria, which is capable to symbiosis with other organisms mutualistically to fulfill nutrition needs. Endophytic bacteria is useful in the fields of agriculture, associated with resistance to pathogens or the ability to form a metabolite that can be transported to each other on endophytic bacteria and plant tissues. One of the endophytic bacterial isolate that successfully collected was Biogen CC-E76 isolate. Strain determination of the endophytic bacteria hasn’t been done. The purpose of this research was to identify endophytic bacteria strain by using 16S rRNA PCR technique, the identifying technique depended on the degree of sequence homology of the small subunit RNA size 16S in diverse bacteria. DNA sequence alignment result (blast) showed that the Biogen CC-E76 isolate is a strain of the bacterium Burkholderia sp. bB24 with homology level of 97%.