| dc.description.abstract | Pacific White shrimp (Litopenaeus vannamei ) is a species of penaid shrimp families that has significant commercial value in the world trade. Fresh feed that have excelent result to increase broodstock gonad maturation of white shrimp is marine worm (Nereis sp), therefore they become WSSV vector. This study aimed to search arternative feed subtituting marine worm as the main feed of white shrimp broodstock. Earthworms (Pheretima sp) have a great potential to be used as an alternative feed to subtituting marine worm for white shrimp broodstock because they can be cultivated, and the nutritional content is quite high. But the content of phospholipids, cholesterol and carotene earthworms were lower than marine worms. The diet treatments were marine worms (Nereis sp) as a control (A), earthworms (Pheretima sp) (B), earthworms enriched by phospholipids, cholesterol and carotene (C) and artificial feed using earthworms flour as the main protein source enriched by phospholipids, cholesterol and carotene (D). The object of this research was virgin white shrimp broodstock. Observed parameters were gonad development stage (GDS) and gonad chemical composition at gonad maturation level II and IV. The results showed that gonad development stage both morfology and histology of C treatment was higher than B and D. The highest of broodstock that reach out for GDS IV was treatment A (28%), followed by treatment C (22.67%) and treatment D (17.33%). The observation of broodstock gonad chemical composition during maturation process from GDS II to GDS IV, showed that although gonad total lipid (TL) of treatments A, C and D increase, only treatment A and treatment C showed an increase in protein content, triacylgliseride, and polar lipid. These affected by betacaroten of both treatments were high enough, so be able to prevent the oxidation of gonad lipid mobilization process. In this study, the treatment that able to produce eggs are treatment A and C. Lipids were preferentially transferred to the eggs, which contained relatively high triacylgliseride and cholesterol levels. The chemical composition of eggs respectively were: protein content of eggs A. 1.87%, C. 12.94%; triacylgliseride content of eggs A. 0.73%, C. 0.70%; cholesterol content of eggs A. 0.62%, C. 0.49%; and beta-caroten content of eggs A. 20.62 mg/1000g, C. 18.2 mg/1000g. It can be concluded that earthworms (Pheretima sp) enriched by phospholipids, cholesterol and beta-carotene can be used as a white shrimp broodstock alternative feed for subtitute marine worms (Nereis sp). | en |
