Eksplorasi Marka Mikrosatelit yang Terkait dengan Sifat Toleransi Keracunan Aluminium pada Padi

Abstract

Acid soils limited agricultural productivity around the world because of the high solubility of aluminum (Al). The objective of this study was to find gene location of Al tolerant trait in rice chromosome from crossed between Cabacu and IR64. One way to found gene location of Al tolerant was by using phenotype analysis and molecular marker microsatellite or SSR (Simple Sequence Repeats). Markers with a specific character could be selected using Bulk Segregant Analysis (BSA). Total 187 strains of F6 rice population from crossed between Cabacu and IR64 were tested using solution of Yoshida. Root length at 45 ppm AlCl3 condition for 21 days was used as data for phenotype linkage analysis with molecular markers. The result of linkage analysis using 47 microsatellite primer shown that RM 104, RM 25, RM 72 and RM 201 were associated with Al stress tolerant trait. These markers shown the genes location which associated with aluminum toxicity tolerance at position 40.166.840 bp chromosome 1, 4.377.460 bp dan 6.762.710 bp chromosome 8 and 20.174.289 bp chromosome 9.