Karakterisasi dan Identifikasi Bakteri Selulolitik Simbion Usus Rayap Macrotermes gilvus.

Abstract

Termites are social insect that have diverse morphology. They have three castes that are reproductives, soldiers, and workers. Macrotermes gilvus belongs to mound builder termite. In their role to digest cellulose, termites use two sources of cellulolytic enzyme, i.e. cellulases produced by the termite , and cellulases produced by the guts symbions. This study was aimed to characterise cellulolytic bacteria of termite hindgut symbionts of worker M. gilvus and to identify the cellulolityc bacteria based on sequences of 16S rRNA gene. Cellulolitic bacteria of termite gut symbionts were isolated and cultured in CMC media. The biochemical characters of bacteria isolats were assayed using Microbact 12A and 12B. Cellulolitic activity was determined based on formation of clear zone index in CMC media bacterial isolats and were tested for biochemical test as well as its cellulase activity based on clear zone. The highest clear zone index isolats genome sequence were analyzed. Four isolates of cellulolitic bacteria were successfully isolated from gut of M. gilvus with aerobic and anaerobic status. The highest formation of clear zone index (2.5) was showed by isolat RA6 isolat. BLAST-N result of 16S rRNA gene sequences of RA6 isolat showed 99% similarity with Paracoccus yeei. This result indicated that RA6 isolat was P. yeei, a cellulolitic bacterium of a termite hindgut of M. gilvus.