Studies of vaccinated broiler chicken serologically and molecular using Infectious Bursal Disease (IBD) vaccine in different doses

Abstract

Disease of Infectious bursal disease (IBD) is a viral disease that causes economic losses to the poultry industry as it causes high mortality and immunosuppression. IBD disease prevention efforts carried out by means of vaccination and biosecurity. IBD vaccination of day old security needs to be investigated its ability to prevent infection. This study aimed to observe the serological response and clinical condition based on examination of molecular actively vaccinated with IBD vaccine at different dose levels of daily life and challenged with virus field isolates. A total of 180 chickens were divided into four groups: K1 (full dose), K2 (½ Dose), K3 (dose ¼) and K4 (Dose 0). The vaccine used was a live IBD vaccine strains intermediate. Observations carried out on five chickens from each group that includes: antibody titers, the ratio bursa weight/bodyweight and pathology images. Presence of field virus infection and the linical condition due to infection challenge was observed by PCR. Observations made on days 1, 7, 28 and 42. Observations showed that the full dose vaccinated chickens started to show positive antibody formation against IBD at day 28 after vaccination, while in chickens vaccinated with a half and a quarter dose positive antibody titers 42 days post-vaccination. Clinical observations showed that vaccinated chickens various dose levels showed the same clinical symptoms than the control group. Similarly to the results of PCR observations it appears that the chickens from the vaccinated group of IBD viruses detected. The results of this study suggest that active IBD day old vaccination is not effective in preventing infection of the field.