Isolasi dan Pemurnian Xantorizol dari Temu Lawak (Curcuma xanthorrhiza Roxb.)

Abstract

Xanthorrhizol is the main active component in the essential oil of temu lawak (Curcuma xanthorriza Roxb.). Xanthorrhizol has antifungal, antibacterial, antioxidant, and anti-inflammatory activities. Xanthorrhizol is isolated by Asriani (2010) using solvent extraction (ethanol 96%), acetylation, Preparative Thin Layer Chromatography (PTLC), and deacetylation, which produce xanthorrhizol with 99% purity. The xanthorrhizol obtained is not pure because there are still other compounds in the mixture. This research perfoms xanthorrhizol isolation using three different modifications based on Asriani’s method. The modified method I conducts two-times extraction: ethanol and hexane extractions. The modified method II conducts three-times extraction: ethanol, hexane, and diethyl ether extractions. The modified method III conducts two-times extraction using ethanol and hexane as solvents, and also conduct PTLC phase II in order to extract the deacetylated product. The modification method I and II produce xanthorrhizol with purity 78.1 and 80.1 % for ethanol extract, and 80.1 and 88.1 % for hexane extract, respectively. Modified method III is able to isolate xanthorrhizol with the highest purity, i.e. 94.3%. GC-MS analysis to the product shows that one of the two peaks appeared at the retention time of 11.44 minutes with an area of 94.3% and MW 218.0 is xanthorrhizol. The results from all of these modified methods were lower than Asriani’s method, which comes to the conclusion that Asriani’s method is better and more efficient.