The Effect of Enriced L-Glutamine Complementary Food on Protein Quality, Cellulair Immunity Profile and Growth of Underweight 6 Month Infants
Sunaryo, Endang S.
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The objective of this study is to develop enriched L-Glutamine Complementary Food (CF) which have desired amino acids and fatty acids performance; to conduct its protein bioavailability and safety assessment; and to investigate the effect of the formula on the growth of underweight 6 months infants and their cellular immunities profile. The enriched L-Glutamine Complementary Food was developed using response surface methodology in order to achieve best performance of amino acids score (methionine and tryptophan) as well as its essential fatty acids. Protein Eficiency Ratio using animal rat experimental was in the range 3.2 * 0.4 (pH.05) and 3.4 ft 0.4 (p<0.05). Additional L-Glutamine by 0.3 gkg body weight or 2.5 gram125 gram CF could enhance better integrity of jejunum in which improve the lymphoid proliferation, thus provide better protection in term of E coli penetration. The experimental study using double blind randomized trial was conducted in 19 villages supervised by medical doctors of 6 Puskesmas in 4 sub districts of Bogor. Three groups of totally 143 infants of 6 months k 1 week were involved in the study, each group were assigned to receive different intervention during 3 months such as MPG (L-Glutamine enriched CF), MPK ( Control CF) and MPP mo L-Glutamine enriched CF). The CF was given daily after reconstituted with boiled water, 7 days a week, 2 packs @ 25g CF per day for 12 weeks (3 months duration). Cellular Immunity Profiles indicated by lymphocytes T, lymphocytes B and natural killer. L-Glutarnine enriched CF (MPG) improved lymphocytes T, T helper and T suppressor better compared to MPK and MPP designated by percentage differences of the cellular immunity profiles between beginning and ending of the intervention. Though protein influenced the blood profiles particularly leucocytes, erythrocytes and haemoglobins development, enrichment L-Glutamine in MPG postulated beneficial improving on Iron Deficiency Anaemia reduction 42,8 % better than 14,2 % in case of MPP. Improvement of the cellular immunity profiles in MPG could reduces morbidity score significantly (p<0.05) by 239 * 302; better than MPP (314 rt 357) and MPK (345 * 468). The enriched L-Glutamine provided OR 0,23 less in morbidity score or have opportunity 4,3 times better in improving immunity. Besides enrichment L-Glutamine CF, morbidity score negative influenced by care giving behaviour (OR 0,46), mother's education (OR 0,43) and intake of CF (OR 0,30). In term of growth, due to improvement of cellular immunity and reducing morbidity, LGlutarnine has indication provided better performance in incremental percentage both weightlage and lengthkge compared to standard during growth monitoring.
- DT - Human Ecology