Pembentukan Diosgenin pada Kultur Kalus Dioscorea bulbifera L.
Diosgenin production by Callus cultures of Dioscorea bulbifera L.
| dc.contributor.author | Tjondronegoro, Puspa D. | |
| dc.date.accessioned | 2010-05-19T04:05:37Z | |
| dc.date.available | 2010-05-19T04:05:37Z | |
| dc.date.issued | 1991 | |
| dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/23114 | |
| dc.description.abstract | Tujuan penelitian adalah mempelajari aspek -aspek kondisi kultur jaringan (kultur kalus) tanaman Dioscorea buLbi fera L. , untuk pembentukan diosgenin secara optimum. Pendekatan permasal ahan di 1 akukan sebagai berikut : (1) mendapatkan sumber eksplan yang berkemampuan mensintesis dan mengakumulasikan di osgeni n. (2) mencari kondisi medium kultur untuk pertumbuhan sel yang optimum. (3) memanipulasi lingkungan kultur dengan perlakuan zat pengatur tumbuh. komponen nutris, pemberian prekursor dan cahaya untuk merangsang biosintesis di osgenin. | |
| dc.description.abstract | The production of diosgenin by in vitro callus cultures of Dioscorea bulbifera L. was investigated. Callus was first initiated from bulbil explant and maintained on solid media of Murashige and Skoog. Different growth rehulators at several level of concentrations were investigated for their ability to maintain cell growth and to stimulate diosgenin formation. Callus grown in the present of 2.4-D and BAP showed consistently better growth and diosgenin production than those grown in media with IAA and NAA. | en |
| dc.publisher | IPB (Bogor Agricultural University) | |
| dc.title | Pembentukan Diosgenin pada Kultur Kalus Dioscorea bulbifera L. | id |
| dc.title | Diosgenin production by Callus cultures of Dioscorea bulbifera L. | en |
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