Profil Metabolit Ekstrak Aseton dan Isolasi Fraksi Bb1 Daun Binahong (Anredera cordifolia) Menggunakan LC-MS/MS

Abstract

Daun binahong (Anredera cordifolia) mengandung berbagai metabolit, tetapi profil metabolit ekstrak aseton dan hasil isolasinya masih terbatas. Penelitian ini bertujuan mengidentifikasi profil metabolit ekstrak aseton serta mengisolasi dan mengarakterisasi fraksi hasil pemurnian. Ekstrak diperoleh melalui maserasi dengan aseton dan dianalisis menggunakan LC-MS/MS. Fraksionasi dan pemurnian dilakukan menggunakan kromatografi cair vakum, kromatografi kolom gravitasi, dan kromatografi lapis tipis preparatif, kemudian hasil pemurnian dianalisis menggunakan LC-MS/MS dan 1H-NMR. Hasil analisis menunjukkan tujuh metabolit yang teridentifikasi secara putatif, yaitu 8-hidroksikuinolin, apigenin, katekin, asam oleanolat, asam oleat, kuersetin-3-ß-D-glukosida, dan asam a-linolenat. Proses pemurnian menghasilkan fraksi Bb1 yang diperkaya komponen dengan karakteristik golongan sitosterol. Berdasarkan analisis LC-MS/MS dan 1H-NMR, komponen utama Bb1 diduga sebagai golongan sitosterol, tetapi fraksi masih mengandung komponen lain sehingga belum dapat dinyatakan sebagai senyawa murni.

Anredera cordifolia leaves contain various metabolites, but information on the metabolite profile of the acetone extract and its isolated components remains limited. This study aimed to identify the metabolite profile of the acetone extract and to isolate and characterize the purified fraction. The extract was obtained by maceration with acetone and analyzed using LC-MS/MS. Fractionation and purification were performed using vacuum liquid chromatography, gravity column chromatography, and preparative thin layer chromatography, followed by LC-MS/MS and 1H-NMR analysis of the purified fraction. The analysis putatively identified seven metabolites, namely 8-hydroxyquinoline, apigenin, catechin, oleanolic acid, oleic acid, quercetin-3-ß-D-glucoside, and a-linolenic acid. The purification process yielded fraction Bb1 enriched with a component exhibiting characteristics of the sitosterol group. Based on LC-MS/MS and 1H-NMR analyses, the major component of Bb1 was suggested to belong to the sitosterol group; however, the fraction still contained other components and therefore could not yet be considered a pure compound.