Evaluasi Metode Analisis Kadar Asam Laktat pada Sabun Cair Menggunakan Kromatografi Cair Kinerja Ultra

Abstract

Sabun cair memiliki komponen bahan aktif kimia yang tidak hanya sekedar surfaktan sebagai bahan utamanya, melainkan dilengkapi dengan bahan-bahan aktif seperti asam laktat yang berfungsi sebagai pengatur keasaman atau pH untuk meningkatkan stabilitas produk sabun cair. Penelitian ini bertujuan untuk mengevaluasi metode analisis asam laktat pada sabun cair menggunakan KCKU berdasarkan parameter validasi. Preparasi sampel dilakukan melalui sonikasi dengan pelarut fase gerak buffer fosfat. Validasi meliputi uji kesesuaian sistem, spesifisitas, linearitas, LOD, LOQ, akurasi, presisi, dan robustness. Hasil analisis spesifisitas menunjukkan tidak terdapat puncak asam laktat pada blanko/placebo, nilai %RSD pada kesesuaian sistem sebesar 0,04%-0,13%, robustness sampel sebesar 0,01%, dan presisi sebesar 1,06% yang memenuhi kriteria =2. Linearitas ditunjukkan pada koefisien korelasi 0,9992 pada rentang 0- 140% b/b. Nilai recovery sebesar 98-101% memenuhi kriteria AOAC. Metode dinyatakan valid dan layak digunakan.

Liquid soap has a component of chemical active ingredients that are not just surfactants as the main ingredient, but are equipped with active ingredients such as lactic acid which functions as an acidity or pH regulator to increase the stability of liquid soap products. This study aims to evaluate the method of analysis of lactic acid in liquid soap using KCKU based on validation parameters. Sample preparation is carried out through sonication with phosphate buffer motion phase solvent. Validation includes testing the suitability of the system, specificity, linearity, LOD, LOQ, accuracy, precision, and robustness. The results of the specificity analysis showed that there was no peak of lactic acid in the blank/placebo, the %RSD value at the suitability of the system was 0.04%-0.13%, the robustness of the sample was 0.01%, and the precision was 1.06% which met the criteria =2. Linearity is shown at a correlation coefficient of 0.9992 in the range of 0-140% b/b. The recovery value of 98-101% met the AOAC criteria. The method is declared valid and suitable for use.