Studi Komparasi Strip Test Metode Fenat dan Sensor Berbasis Antosianin untuk Analisis Amonia dalam Limbah Cair

Abstract

FATIYA NUR ADILLA. Studi Komparasi Strip Test Metode Fenat dan Sensor Berbasis Antosianin untuk Analisis Amonia dalam Limbah Cair. Dibimbing oleh DIMAS ANDRIANTO.

Limbah cair berpotensi mengandung amonia yang toksik bagi lingkungan. Penelitian bertujuan mengembangkan strip test deteksi cepat amonia berbasis metode fenat serta antosianin bunga telang sebagai metode skrining awal. Hasil warna dianalisis dengan ImageJ melalui metode analisis RGB. Kondisi optimum diperoleh waktu reaksi 5 menit dan volume pereaksi 50 µL untuk metode fenat, waktu reaksi 1 menit dan konsentrasi NaOH 0,01 M untuk antosianin, dengan channel green sebagai channel optimum. Validasi strip test fenat memiliki nilai R2 = 0,9974, % recovery = 85,54-91,72%, %RSD = 2,49%, LOD dan LOQ sebesar 0,0193 dan 0,0584 mg/L, metode dinyatakan valid dan memenuhi persyaratan AOAC 2016. Sensor antosianin didapatkan nilai R2 = 0,99531, % recovery 60,73-150,47%, nilai RSD 6,80%, LOD dan LOQ sebesar 0,7741 dan 2,3457 mg/L, sehingga dinyatakan tidak valid terhadap standar AOAC 2016. Verifikasi terhadap metode spektrofotometri UV-Vis menunjukkan tidak terdapat perbedaan yang signifikan (p-value 0,639 > 0,05), sehingga simpulan riset ini yaitu strip test fenat berpotensi menjadi metode skrining awal amonia dalam limbah cair.

Kata kunci : Amonia, antosianin, ImageJ, spektrofotometri, strip test

FATIYA NUR ADILLA. Comparative Study of Phenate Test Strip Method and Anthocyanin-Based Sensor for Ammonia Analysis in Liquid Waste. Supervised by DIMAS ANDRIANTO.

Liquid waste has potential to contain ammonia, which is toxic to the environment. This study developed a rapid ammonia detection test strip based on the phenate method and anthocyanin from butterfly pea flowers as an initial screening method. The resulting color analyzed using ImageJ software through RGB analysis. Optimum conditions were 5 minutes reaction time and 50 µL reagent volume for the phenate method, 1 minute reaction time and 0.01 M NaOH concentration for anthocyanin method, with the green channel as the optimum channel. The validation results of the phenate test strip have an R2 value = 0.9974, % recovery = 85.54 -91.72%, %RSD value of 2.49%, LOD and LOQ of 0.0193 and 0.0584 mg/L, the method is declared valid and meets the requirements of AOAC 2016. The anthocyanin test strip obtained an R2 value = 0.99531, %recovery 60.73-150.47%, RSD value of 6.80%, LOD and LOQ of 0.7741 and 2.3457 mg/L, so it is declared invalid against the AOAC 2016 standard. Verification of UV-Vis spectrophotometry method showed no significant difference (p-value 0.639 > 0.05), so phenate test strip has the potential to be an initial screening method for ammonia in liquid waste.

Keywords: Ammonia, anthocyanin, ImageJ, spectrophotometry, test strip