| dc.description.abstract | Kucing sebagai hewan peliharaan yang hidup dekat dengan manusia berpotensi
menjadi sumber penyebaran bakteri resistan antibiotik, termasuk Escherichia coli. Selain
resistansi, pembentukan biofilm perlu diperhatikan karena meningkatkan ketahanan bakteri
terhadap tekanan lingkungan, termasuk antibiotik. Tujuan penelitian ini adalah untuk
mengidentifikasi multidrug resistance pada isolat Escherichia coli asal kucing, mengetahui
aktivitas pembentukan biofilm pada isolat Escherichia coli yang diperoleh, dan
menganalisis hubungan antara sifat multidrug resistance dengan kemampuan pembentukan
biofilm. Sebanyak 15 isolat diuji menggunakan metode difusi cakram Kirby-Bauer untuk
sensitivitas antibiotik (Ampisillin, Aztreonam, Penisillin G, Metronidazol, Gentamisin,
Tetrasiklin, Enrofloksasin) dan metode Congo Red Agar untuk deteksi biofilm. Hasil
menunjukkan 7 dari 15 isolat tergolong multidrug resistance. Enam dari 15 isolat mampu
membentuk biofilm yang ditandai koloni berwarna gelap dan bertekstur kering. Analisis
uji Fisher’s Exact Test menunjukkan tidak ada hubungan signifikan antara multidrug
resistance dan pembentukan biofilm (p>0,05). Disimpulkan bahwa Escherichia coli
menunjukkan kemampuan sebagai bakteri multidrug resistance dan mampu membentuk
biofilm. Tidak terdapat hubungan yang signifikan antara multidrug resistance dan
pembentukan biofilm.
Cats, as pets that live close to humans, have the potential to become a source of
antibiotic-resistant bacteria, including Escherichia coli. In addition to resistance, biofilm
formation is important to consider because it increases bacterial resistance to environmental
stress, including antibiotics. The purpose of this study was to identify multidrug resistance
in Escherichia coli isolates from cats, determine the biofilm-forming activity of the
obtained Escherichia coli isolates, and analyze the relationship between multidrug
resistance and biofilm-forming ability. A total of 15 isolates were tested using the Kirby-
Bauer disc diffusion method for antibiotic sensitivity (Ampicillin, Aztreonam, Penicillin G,
Metronidazole, Gentamicin, Tetracycline, Enrofloxacin) and the Congo Red Agar method
for biofilm detection. The results showed that 7 of the 15 isolates were classified as
multidrug resistant because they were resistant to three or more classes of antibiotics. 6 of
the 15 isolates were able to form biofilms, as indicated by dark-colored colonies and a dry
texture. Fisher's Exact Test analysis showed no significant relationship between multidrug
resistance and biofilm formation (p>0.05). It was concluded that Escherichia coli exhibited
multidrug resistance and the ability to form biofilms, yet there was no significant
relationship between multidrug resistance and biofilm formation. | |
